AI Article Synopsis
- Beclin-1, traditionally linked to macroautophagy and tumor processes, has a new, independent role in helping chromosomes align properly during cell division (mitosis).
- When Beclin-1 is reduced in HeLa cells, crucial proteins for chromosome congression are diminished, leading to significant mitotic issues.
- The study reveals that Beclin-1 interacts directly with Zwint-1, a key component of the KMN complex, thereby enhancing the recruitment of outer kinetochore proteins essential for proper attachment of chromosomes to the spindle fibers.
Article Abstract
The functions of Beclin-1 in macroautophagy, tumorigenesis and cytokinesis are thought to be mediated by its association with the PI3K-III complex. Here, we describe a new role for Beclin-1 in mitotic chromosome congression that is independent of the PI3K-III complex and its role in autophagy. Beclin-1 depletion in HeLa cells leads to a significant reduction of the outer kinetochore proteins CENP-E, CENP-F and ZW10, and, consequently, the cells present severe problems in chromosome congression. Beclin-1 associates with kinetochore microtubules and forms discrete foci near the kinetochores of attached chromosomes. We show that Beclin-1 interacts directly with Zwint-1-a component of the KMN (KNL-1/Mis12/Ndc80) complex-which is essential for kinetochore-microtubule interactions. This suggests that Beclin-1 acts downstream of the KMN complex to influence the recruitment of outer kinetochore proteins and promotes accurate kinetochore anchoring to the spindle during mitosis.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3615652 | PMC |
| http://dx.doi.org/10.1038/embor.2013.23 | DOI Listing |
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