AI Article Synopsis
- A system was developed to display antigens from Enterovirus type 71 (EV71) on the surface of Lactococcus lactis for potential use as an oral vaccine.
- The viral capsid protein (VP1) gene was used to create fusion proteins, which were expressed in E. coli and then introduced to L. lactis.
- Mice fed with L. lactis displaying these proteins showed an immune response, marking this as the first instance of an EV71 antigen on a food-grade organism, which may lead to new vaccine strategies and flexible protein presentations using L. lactis.
Article Abstract
In this study, we have developed a system for display of antigens of Enterovirus type 71 (EV71) on the cell surface of L. lactis. The viral capsid protein (VP1) gene from a local viral isolate was utilized as the candidate vaccine for the development of oral live vaccines against EV71 using L. lactis as a carrier. We expressed fusion proteins in E. coli and purified fusion proteins were incubated with L. lactis. We confirmed that mice orally fed with L. lactis displaying these fusion proteins on its surface were able to mount an immune response against the epitopes of EV71. This is the first example of an EV71 antigen displayed on the surface of a food grade organism and opens a new perspective for alternative vaccine strategies against the EV71. We believe that the method of protein docking utilized in this study will allow for more flexible presentations of short peptides and proteins on the surface of L. lactis to be useful as a delivery vehicle.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3586462 | PMC |
| http://dx.doi.org/10.1155/2013/431315 | DOI Listing |
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