AI Article Synopsis
Article Abstract
RNA-binding proteins are at the heart of posttranscriptional gene regulation, coordinating the processing, storage, and handling of cellular RNAs. We show here that GRSF1, previously implicated in the binding and selective translation of influenza mRNAs, is targeted to mitochondria where it forms granules that colocalize with foci of newly synthesized mtRNA next to mitochondrial nucleoids. GRSF1 preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long noncoding RNAs for cytb and ND5, each of which contains multiple consensus binding sequences. RNAi-mediated knockdown of GRSF1 leads to alterations in mitochondrial RNA stability, abnormal loading of mRNAs and lncRNAs on the mitochondrial ribosome, and impaired ribosome assembly. This results in a specific protein synthesis defect and a failure to assemble normal amounts of the oxidative phosphorylation complexes. These data implicate GRSF1 as a key regulator of posttranscriptional mitochondrial gene expression.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.cmet.2013.02.006 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Regulating the regulators: long non-coding RNAs as autophagic controllers in chronic disease management.
J Biomed Sci
December 2024
Cancer Biology Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati (IITG), Guwahati, Assam, 781039, India.
The increasing prevalence of chronic diseases and their associated morbidities demands a deeper understanding of underlying mechanism and causative factors, with the hope of developing novel therapeutic strategies. Autophagy, a conserved biological process, involves the degradation of damaged organelles or protein aggregates to maintain cellular homeostasis. Disruption of this crucial process leads to increased genomic instability, accumulation of reactive oxygen species (ROS), decreased mitochondrial functions, and suppression of ubiquitination, leading to overall decline in quality of intracellular components.
View Article and Find Full Text PDFGenetic architecture of RNA editing, splicing and gene expression in schizophrenia.
Hum Mol Genet
December 2024
Bioinformatics Interdepartmental Program, University of California, Los Angeles, 611 Charles E. Young Drive East, Los Angeles, CA 90095-1570, United States.
Genome wide association studies (GWAS) have been conducted over the past decades to investigate the underlying genetic origin of neuropsychiatric diseases, such as schizophrenia (SCZ). While these studies demonstrated the significance of disease-phenotype associations, there is a pressing need to fully characterize the functional relevance of disease-associated genetic variants. Functional genetic loci can affect transcriptional and post-transcriptional phenotypes that may contribute to disease pathology.
View Article and Find Full Text PDFDRBD18 acts as a transcript-specific RNA editing auxiliary factor in Trypanosoma brucei.
RNA
December 2024
Department of Microbiology and Immunology, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, New York, 14203, USA
Uridine insertion/deletion (U-indel) RNA editing of mitochondrial transcripts is a posttranscriptional modification in kinetoplastid organisms, resulting in the generation of mature mRNAs from cryptic precursors. This RNA editing process involves a multi-protein complex holoenzyme and multiple accessory factors. Recent investigations have highlighted the pivotal involvement of accessory RNA binding proteins (RBPs) in modulating RNA editing in T.
View Article and Find Full Text PDFPentatricopeptide repeat proteins in plants: Cellular functions, action mechanisms and potential applications.
Plant Commun
December 2024
Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao 266237, China. Electronic address:
Pentatricopeptide repeat (PPR) proteins are involved in nearly all aspects of post-transcriptional processing in plant mitochondria and plastids, where they play a vital role in plant growth, development, cytoplasmic male sterility (CMS) restoration, and response to biotic and abiotic stresses. Through research in the last three decades, PPR functions and the primary mechanisms by which PPR proteins mediate post-transcriptional processing have been uncovered. Here, we aim to summarize the advances in PPR research with highlighting on the mechanisms of how PPR proteins mediate RNA editing, intron splicing, and RNA maturation in the context of their role in organellar gene expression.
View Article and Find Full Text PDFThe USP11/Nrf2 positive feedback loop promotes colorectal cancer progression by inhibiting mitochondrial apoptosis.
Cell Death Dis
December 2024
Department of Gastroenterology, Zhongnan Hospital of Wuhan University, Wuhan, China.
Abnormal antioxidant capacity of cancer is closely related to tumor malignancy. Modulation of oxidative stress status is a novel anticancer therapeutic target. Nrf2 is a key regulator of various antioxidant enzymes, but the mechanism of its deubiquitination remains largely unclear.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!