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In vitro antifungal activity of synthetic dsRNA molecules against two pathogens of banana, Fusarium oxysporum f. sp. cubense and Mycosphaerella fijiensis. | LitMetric

AI Article Synopsis

  • A study was conducted to evaluate the effectiveness of synthetic double-stranded RNA (dsRNA) molecules in inhibiting the spore germination of two major banana pathogens: Fusarium oxysporum and Mycosphaerella fijiensis.
  • The results showed that the dsRNAs could significantly silence target genes and inhibit spore germination, with varying inhibition rates depending on the type of dsRNA and the pathogen it was applied to.
  • Specifically, dsRNAs targeting adenylate cyclase and DNA polymerases were identified as the most effective candidates for developing RNA-based protection strategies against these fungal threats in banana crops.

Article Abstract

Background: A key challenge for designing RNAi-based crop protection strategies is the identification of effective target genes in the pathogenic organism. In this study, in vitro antifungal activities of a set of synthetic double-stranded RNA molecules on spore germination of two major pathogenic fungi of banana, Fusarium oxysporum Schlecht f. sp. cubense WC Snyder & HN Hans (Foc) and Mycosphaerella fijiensis Morelet (Mf) were evaluated.

Results: All the tested synthetic dsRNAs successfully triggered the silencing of target genes and displayed varying degrees of potential to inhibit spore germination of both tested banana pathogens. When Foc dsRNAs were applied to Foc spores, inhibition ranged from 79.8 to 93.0%, and from 19.9 to 57.8% when Foc dsRNAs were applied to Mf spores. However, when Mf dsRNAs were applied on Mf spores, inhibition ranged from 34.4 to 72.3%, and from 89.7 to 95.9% when Mf dsRNAs were applied to Foc spores.

Conclusion: The dsRNAs for adenylate cyclase, DNA polymerase alpha subunit and DNA polymerase delta subunit showed high levels of spore germination inhibition during both self- and cross-species tests, making them the most promising targets for RNA-mediated resistance in banana against these fungal pathogens. © 2013 Society of Chemical Industry.

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Source
http://dx.doi.org/10.1002/ps.3480DOI Listing

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