To validate an LC-MS/MS method for simultaneous determination of deoxynivalenol (DON) and its acetylated derivatives, 3-acetyl-deoxynivalenol (3ADON) and 15-acetyl-deoxynivalenol (15ADON), in wheat using a multifunctional column, an inter-laboratory study was performed in 9 laboratories using one blank wheat sample, three spiked wheat samples (10, 50, 150 µg/kg) and one naturally contaminated wheat sample. The recoveries ranged from 98.8 to 102.6% for DON, 89.3 to 98.7% for 3ADON, and from 84.9 to 90.0% for 15ADON. The relative standard deviations for repeatability (RSDR) and reproducibility (RSDR) of DON were in the ranges of 7.2-11.3% and 9.5-22.6%, respectively. For 3ADON, the RSDR ranged from 5.3 to 9.5% and the RSDR ranged from 16.1 to 18.0%, while for 15ADON, the RSDR ranged from 6.2 to 11.2% and the RSDR ranged from 17.0 to 27.2%. The HorRat values for the three analytes ranged from 0.4 to 1.2. These results validate this method for the simultaneous determination of DON and its acetylated derivatives, 3ADON and 15ADON.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.3358/shokueishi.54.75 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Development and Application of a New QuEChERS Method Coupled with UPLC-QTOF-MS/MS for Analysis of Tiafenacil and Its Photolysis Products in Water.
J Agric Food Chem
December 2024
College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.
This research centered on the novel pyrimidinedione herbicide, tiafenacil. Residues of tiafenacil and its three photolysis products (PP1 to PP3) in water were analyzed using advanced QuEChERS and UPLC-QTOF-MS/MS techniques, reaching a low limit of quantitation (LOQ) of 10 μg/L. Calibration curves exhibited a high degree of linearity ( ≥ 0.
View Article and Find Full Text PDFA cyanide-free sample preparation methodology prior to determination of vitamin B in infant milk formula using hydrophilic interaction liquid chromatography with fluorescence detection.
Talanta
January 2025
School of Science, University of Waikato, Private Bag 3105, Hamilton, 3240, New Zealand. Electronic address:
The analysis of vitamin B in infant formulas typically requires the use of cyanide during sample preparation to convert the unstable vitamers (hydroxocobalamin, methylcobalamin and adenosylcobalamin) to cyanocobalamin, the most stable form of vitamin B. To eliminate the risk to laboratory analysts in handling cyanide, alternative strategies are preferred for the analysis of vitamin B. This research demonstrates the use of cobalamin-derived α-ribazole (a nucleoside moiety of vitamin B) to determine total vitamin B content.
View Article and Find Full Text PDFA novel organic chromo-fluorogenic optical sensor for detecting chromium ions.
Heliyon
September 2024
Chemistry Department, Faculty of Science, Suez Canal University, Ismailia, Egypt.
Sensing trivalent chromium ion (Cr(III)) is widely applied in different areas, such as clinical analysis, marine, environmental monitoring, or even chemical industry applications. Cr(III) has a significant role in the physiological process of human life. It is classified as an essential micronutrient for living organisms.
View Article and Find Full Text PDFDetermination of Taurine in Infant Formula and Adult/Pediatric Nutritionals by Ultra High-Performance Liquid Chromatography with Multiple Reaction Monitoring-Mass Spectrometry: Single-Laboratory Validation, First Action 2022.08.
J AOAC Int
November 2024
FrieslandCampina, Stationsplein 4, 3818 LE, Amersfoort, the Netherlands.
Background: Taurine (2-aminoethanesulfonic acid) is an essential β-amino acid, which is one of the most abundant intracellular amino acid component in humans. The level of free taurine in human milk is higher than in bovine milk. Since taurine is considered important for the development of a newborn, fortification of infant nutrition with taurine is common.
View Article and Find Full Text PDFQuantification of Total Sulfites in Shrimp by BIOFISH 300/3000 SUL Method, Collaborative Study: Final Action 2021.09.
J AOAC Int
November 2024
BIOLAN Microbiosensores S.L., Parque Tecnológico de Bizkaia, Laida Bidea 409, 48170 Zamudio, Bizkaia, Spain.
Background: In December 2021, the BIOFISH 300 SUL method for the determination of total sulfites in shrimp was adopted as a First Action Official Method of AnalysisSM by the AOAC INTERNATIONAL.
Objective: A collaborative study was conducted in February 2023 in order to test the reproducibility of the method.
Methods: The method is based on the use of a benchtop biosensor device that relates the concentration of sulfite to a quantifiable electric current signal.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!