A newly designed apparatus for anaerobic measurement of blood pH at low temperatures is presented. This apparatus features a larger glass electrode surface area, which is necessary to overcome the increased resistance of the glass as blood pH determinations at 0-15 C. are undertaken. Utilization of this equipment has confirmed the deltapH per degree C. of whole blood to be 0.0156, while intracellular pH of blood has a deltapH per degree C. of 0.0165.
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http://dx.doi.org/10.1093/ajcp/63.2.264 | DOI Listing |
Protoplasma
January 2025
Laboratory of Cytology of Unicellular Organisms, Institute of Cytology of the Russian Academy of Sciences, St. Petersburg, 194064, Russia.
The representatives of the archamoebian genus Pelomyxa are amoeboid anaerobic protists that inhabit fresh-water anoxic sediments, and most of them are usually multinucleate. The cytoplasm of these unicellular organisms is highly complicated and contains numerous vacuoles of different types, as well as a wide range of prokaryotic endocytobionts, agglomerations of glycogen, lipids, etc. Among the great variety of cytoplasmic structures in P.
View Article and Find Full Text PDFMicroorganisms
November 2024
School of Environmental Studies, China University of Geosciences (Wuhan), Wuhan 430078, China.
Iron, Earth's most abundant redox-active metal, undergoes both abiotic and microbial redox reactions that regulate the formation, transformation, and dissolution of iron minerals. The electron transfer between ferrous iron (Fe(II)) and ferric iron (Fe(III)) is critical for mineral dynamics, pollutant remediation, and global biogeochemical cycling. Bacteria play a significant role, especially in anaerobic Fe(II) oxidation, contributing to Fe(III) mineral formation in oxygen-depleted environments.
View Article and Find Full Text PDFBiosensors (Basel)
November 2024
Department of Chemistry, University of Waterloo, 200 University Ave West, Waterloo, ON N2L 3G1, Canada.
Hypoxia, characterized by inadequate tissue oxygenation, may result in tissue damage and organ failure if not addressed. Current detection approaches frequently prove insufficient, depending on symptoms and rudimentary metrics such as tissue oxygenation, which fail to comprehensively identify the onset of hypoxia. The European Pressure Ulcer Advisory Panel (EPUAP) has recognized sweat lactate as a possible marker for the early identification of decubitus ulcers, nevertheless, neither sweat lactate nor oxygenation independently provides an appropriate diagnosis of hypoxia.
View Article and Find Full Text PDFBiotechnol Bioeng
December 2024
Department of Microbiology, Hubei Key Laboratory of Cell Homeostasis, College of Life Science, TaiKang Center for Life and Medical Sciences, Wuhan University, Wuhan, China.
Photosynthesis, the most important biological process on Earth, converts light energy into chemical energy with essential pigments like chlorophylls and bacteriochlorophylls. The ability to reconstruct photosynthesis in heterotrophic organisms could significantly impact solar energy utilization and biomass production. In this study, we focused on constructing light-dependent biosynthesis pathways for bacteriochlorophyll (BChl) a and bacteriochlorophyllide (BChlide) d and c in the model strain Escherichia coli.
View Article and Find Full Text PDFMicroorganisms
November 2024
Istituto per la Bioeconomia, Consiglio Nazionale delle Ricerche, Via Madonna del Piano 10, Sesto Fiorentino, 50019 Firenze, Italy.
Background: Dark anaerobiosis promotes the acidification of the thylakoid lumen and a reduction in the plastoquinone (PQ) pool. The relationship between the reduction in the PQ pool in the dark and the induction of the xanthophyll cycle under high light stress was investigated in .
Methods: To achieve a comprehensive oxidative/reductive (aerobic/anaerobic conditions) state of the PQ pool, cultures were bubbled with air or nitrogen for 4 h.
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