[Effect of coenzyme Q10 supplementation on apoptosis, free radical oxidative processes and proteome pool of microsomal and cytosol fractions of hepatocytes using food ration with different lipid component].

Our aim was to analyze biochemical mechanism in regulation and adaptation to feeding with omega-3 and omega-6 PUFA plus coenzyme Q10-based diet in rats. The rats were supplemented with Co Q10 (100 mg/kg b.wt.) for 12 month. Male rats were fed a control, control + Q10, fish oil + Q10 palm oil + Q10 and flax oil + Q10 diet. The learning and tests were monitored in 1st, 3rd, 6th and 12th month after the start of feeding. There was studied the content of lipid peroxidation products in the liver, the level of their potential substrates polyunsaturated fatty acids of omega-3 and omega-6 families and MEC (metabolism effectiveness coefficient) index value. Growth of relative number of hepatocyte in the state of late apoptosis has been detected in rats receiving fish oil, linseed oil and palm oil as compared to the control group at the age of 1 and 3 months. In later period no differences in the invested indices, that can be the result of impact of CoQ10 and adaptation of organism to diet. Proteomic studies of microsomal fraction of hepatocytes in rats have found differences in the manifestation of catalase and cetochrome b5, associated both with age of animals and type of fat in diet. Proteomic analysis of cytosolic fraction revealed the expression of c-type lectin in the later stages of ontogeny. The levels of lipid peroxidation markers obtain to minimum at 3 month in serum and at 6 month in liver. The specific proteomic changes of influence of diet composition were identified during the research.