AI Article Synopsis
- The study aims to optimize the extraction conditions of mycelium polysaccharides from selenium-rich Morchella esculenta using neutral proteinase to increase yield.
- Researchers used single factor tests and orthogonal experiment design to assess factors like enzyme dosage, time, temperature, and material/liquid ratio on extraction efficiency.
- The best extraction conditions were found to be 1.5% enzyme dosage, 2 hours at 40°C, and a 1:15 material/liquid ratio, achieving an extraction rate of 11.26%, indicating a simple and effective process.
Article Abstract
Objective: To study the extraction conditions of mycelium polysaccharides from selenium-rich Morchella esculenta with neutral proteinase for increasing of yield.
Methods: On the basis of single factor tests, orthogonal experiment design was applied to analyze the influence of factors including enzyme dosage, enzymolysis time, enzymolysis temperature and material/liquid ratio on the extraction rate of the polysaccharides.
Results: The optimal extraction conditions of mycelium polysaccharides from selenium-rich Morchella esculenta was as follows: enzyme dosage was 1.5%, enzymolysis time was 2 h, enzymolysis temperature was 40 degrees C, and material/liquid ratio was 1:15. The extraction rate of polysaccharide was 11.26% under the extraction conditions.
Conclusion: The process is simple, stable and practicable, and can be used for the extraction of mycelium polysaccharides from selenium-rich Morchella esculenta.
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