Int J Pept Protein Res
Laboratory of Peptide Chemistry, Academy of Sciences of the USSR, Pushchino, Moscow Region.
Published: April 1990
Peptide synthesis catalysed by papain was studied using thio-alpha-amino acids (S-acids) as a carboxyl component. It was found, for example, that with Z-AlaSH (pK 2.70) the maximal yield of the peptide Z-AlaValNH2 was obtained at pH 8-8.5. A two-fold excess of Z-AlaSH furnished peptides with yields close to 100%. Thio-amino acids with bulky side groups, for example, Z-IleSH, Z-Asp(OBu')SH, gave peptides with a low yield. Papain interacts with Z-AlaSH better than do bromelain or ficin.
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http://dx.doi.org/10.1111/j.1399-3011.1990.tb00060.x | DOI Listing |
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