AI Article Synopsis
- The study aimed to quickly identify the bla KPC gene in 38 clinical isolates of Klebsiella pneumoniae that showed reduced susceptibility to carbapenems using methods like the modified Hodge Test (MHT) and a specialized real-time assay (NASBA™).
- Thirty-two out of 38 isolates tested positive for carbapenemase production with MHT, and all strains displayed KPC production in further tests.
- The detection of the bla KPC gene was successful in all isolates, confirming the effectiveness of rapid identification methods, highlighting the urgent need for such techniques in clinical settings to address K. pneumoniae infections.
Article Abstract
Background: The aim of this study was the rapid identification of bla KPC gene in 38 Klebsiella pneumoniae clinical isolates with reduced susceptibility to carbapenems. The modified Hodge Test (MHT) was carried out to phenotypically determine whether resistance to carbapenems was mediated by a carbapenemase. The detection of the bla KPC gene was performed by real-time acid nucleic sequence-based amplification (NASBA™™), specifically designed for the detection of KPC RNA target.
Results: Thirty-two/38 isolates evaluated by MHT showed the production of carbapenemases, while all the strains exhibited the production of KPC by inhibition test with phenylboronic acid (the combined disk test with IPM/IPM plus phenylboronic acid). The detection of bla KPC gene by Nuclisens EasyQ KPC yielded positive results in 38/38 (100%) strains. The presence of bla KPC gene was confirmed in all K. pneumoniae isolates when tested by the gold standard PCR assay.
Conclusions: In consideration of the serious challenge represented by infections due to K. pneumoniae it appears necessary the rapid identification of carbapenemases in clinical settings as it is made possible by the use of NASBA™ assay.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3581762 | PMC |
| http://dx.doi.org/10.1186/2193-1801-2-31 | DOI Listing |
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