AI Article Synopsis
- The study aimed to identify virulence and bacteriocin genes in 78 strains of E. faecalis isolated from water wells, along with their antimicrobial susceptibility.
- The researchers used PCR to amplify genetic material and performed agar diffusion tests to assess antibiotic resistance, finding that 93.5% of strains had the aggA virulence gene, while entA was the most common bacteriocin gene at 64.1%.
- The analysis revealed significant variability in virulence and bacteriocin genes among strains, with a notable prevalence of resistance to tetracycline and chloramphenicol, and a statistical link between certain genetic profiles and bacterial resistance.
Article Abstract
The objectives of this study were to detect genes for virulence and bacteriocins in addition to studying the antimicrobial susceptibility of 78 strains of E. faecalis isolated from water wells for human consumption. The virulence and bacteriocin genes of 78 E. faecalis were amplified by PCR and visualized in agarose gels. The antimicrobial susceptibility was determined through diffusion agar tests and the MIC through microdilution. It was observed that the major percentage of virulence genes in the E. faecalis strains corresponds to aggA (93.5%). The bacteriocin gene entA (64.1%) is the most frequently detected. The studied strains exhibited different virulence and bacteriocin genes, and an important antibacterial resistance. The most common resistant phenotype (n = 14) corresponds to tetracycline and chloramphenicol and the less frequent (n = 2) to ciprofloxacin and moxifloxacin. Eight different genetic profiles were observed for virulence y bacteriocin genes. It was determined a statistical association between the bacterial resistance and some of the genetic profiles detected.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3579423 | PMC |
| http://dx.doi.org/10.1186/2193-1801-2-43 | DOI Listing |
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