Intracellular pH (pHi) of mouse lymphoid leukemia P388 cells was measured at the wavelength of 518 and 570 nm of fluorescein: pHi of rubomycin-sensitive P388 cells was higher than pHi of the cells resistant to this antitumour antibiotic (6.98 +/- 0.04 and 6.63 +/- 0.03, respectively, P less than 0.001). The role of pHi is established during induction of the resistance of tumour cells to the antitumour drugs.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Cryptoic acids A and B, benzene-containing polyketides, and cyclocryptamide, a modified diketopiperazine, from an actinomycete of the genus Cryptosporangium.
J Antibiot (Tokyo)
January 2025
Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, Toyama, Japan.
Two new benzene-containing polyketides, cryptoic acids A (1) and B (2), along with a new acylated diketopiperazine designated cyclocryptamide (3), were isolated from the culture extract of Cryptosporangium sp. YDKA-T02. The absolute configuration of amino acid components in 3 was determined by Marfey's method.
View Article and Find Full Text PDFWychimicins E and F from a rare actinomycete of the genus Cryptosporangium.
J Antibiot (Tokyo)
December 2024
Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University 5180 Kurokawa, Imizu, Toyama, 939-0398, Japan.
Two new spirotetronate class compounds designated wychimicins E (1) and F (2) were isolated from the culture extract of an actinomycete Cryptosporangium sp. RD061707. Their structures were determined through extensive NMR analysis in comparison with wychimicin C.
View Article and Find Full Text PDFSporangimicins A-D, acylated maltose derivatives from a rare actinomycete of the genus Pseudosporangium.
J Antibiot (Tokyo)
November 2024
Biotechnology Research Center, 5180 Kurokawa, Imizu, Toyama, 939-0398, Japan.
Sporangimicins A-D (1-4), four anomeric pairs of diacyl disaccharides that represent a new metabolite class, were discovered from the culture extract of an actinomycete Pseudosporangium sp. RD061809. Compounds 1-4 caused peak separation in the HPLC chromatogram and partial duplication of the NMR resonances by anomeric interconversion of a maltose core modified at the two sugar 6-positions with an isobutanoyl and a methyl-branched long-chain dienoyl groups.
View Article and Find Full Text PDFSynthesis and Anti-Cancer Activity of the Novel Selective Glucocorticoid Receptor Agonists of the Phenylethanolamine Series.
Int J Mol Sci
August 2024
Department of Chemical Carcinogenesis, Institute of Carcinogenesis, N.N. Blokhin National Medical Research Center for Oncology, Kashirskoe Shosse 24-15, Moscow 115478, Russia.
Article Synopsis
- Glucocorticoids (GCs) are commonly used to treat blood cancers but can cause various side effects due to how they interact with glucocorticoid receptors (GRs).
- Selective GR agonists (SEGRAs) like CpdA aim to enhance the beneficial anticancer effects while minimizing side effects; however, CpdA faces challenges with chemical instability.
- The newly developed derivative, CpdA-03, shows improved stability and GR affinity, demonstrating significant anticancer activity in lymphoma models, with a tripling reduction in tumor volume compared to conventional treatments.
Nrf2-Regulated Antioxidant System in Cells of In Vivo Drug-Resistant P388 Murine Leukemia Strains.
Bull Exp Biol Med
July 2024
Federal Research Center of Problems of Chemical Physics and Medicinal Chemistry, Russian Academy of Sciences, Chernogolovka, Moscow Region, Russia.
We studied the expression of Nrf2 transcription factor and antioxidant system proteins in drug-resistant murine leukemia strains P388 in vivo, as well as the redox status of cells under conditions of induced oxidative stress. Immunoblotting and real-time PCR showed that the cyclophosphamide-resistant strain P388 (P388/CP) exhibits Nrf2-mediated drug resistance. Cells of the P388/CP strain are characterized by high expression of Nrf2, which leads to a significant increase in the expression of ARE genes and antioxidant system proteins, as well as to the effective maintenance of redox homeostasis under conditions of induced oxidative stress.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!