AI Article Synopsis
- Researchers expressed porcine ZP3 and ZP4 in E. coli to provide a source of purified native zona pellucida glycoproteins for use in immunocontraception.
- The proteins were characterized through SDS-PAGE and Western blot, and their effectiveness in inducing immune responses and reducing fertility was tested in female mice.
- Results showed that immunization with these recombinant proteins led to strong antibody production, inhibited fertilization, and significantly decreased fertility, indicating their potential for a contraceptive vaccine development.
Article Abstract
Problem: To overcome availability of the purified native zona pellucida (ZP) glycoproteins for immunocontraception, porcine ZP3, and ZP4 were expressed in E. coli.
Method Of Study: Purified recombinant proteins were characterized by SDS-PAGE and Western blot, and immunogenicity and contraceptive efficacy determined in FvB/J female mice.
Results: Purified ZP3, ZP3 with promiscuous T-cell epitope of tetanus toxoid, ZP4 and ZP4 incorporating promiscuous T-cell epitope of bovine RNase revealed ~44-, ~49-, ~53-, and ~55-kDa bands by SDS-PAGE and Western blot, respectively. Immunization of female mice with recombinant proteins elicited high antibody titers as well as T-cell responses. Immune sera recognized mouse oocyte ZP and also inhibited in vitro fertilization. Immunized mice showed significant decrease in fertility. Recombinant proteins were able to recall memory antibody response in female mice primed with porcine native ZP.
Conclusion: Availability of recombinant porcine proteins will be useful in the development of contraceptive vaccine.
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| http://dx.doi.org/10.1111/aji.12095 | DOI Listing |
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