This study evaluated the efficacy of a PCR-based system (DuPont Qualicon BAX) for detection of Salmonella in sprouts and spent irrigation water collected during sprouting of seeds naturally contaminated with Salmonella. Alfalfa seeds were grown in Mason jars at 20 and 30°C for 3 days. Levels of Salmonella present in the water and sprouts were determined by most-probable-number (MPN) analysis. Background microflora levels were also determined. Samples of spent irrigation water and sprouts were enriched overnight individually in tetrathionate broth and in buffered peptone water with novobiocin at 42°C and then run in the BAX system. Samples were also enriched according to the U.S. Food and Drug Administration's Bacteriological Analytical Manual (FDA BAM) method for Salmonella as a comparison. Salmonella levels were lower at 20°C compared with 30°C for some trials, and background microflora levels ranged from 10(7) to 10(8) CFU/g or ml at 20°C and 10(8) to 10(9) CFU/g or ml at 30°C. In trials with a Salmonella level >1.1 MPN/g or ml, both the BAX and FDA BAM methods were able to detect Salmonella in all samples. In trials with lower levels (0.21 MPN/g or ml or lower) of Salmonella, BAX was able to detect more positive samples than FDA BAM. For one trial with <0.003 MPN/g or ml of Salmonella, the presence of the pathogen was not indicated by either the BAX or the FDA BAM method. The results suggest that PCR detected low levels of Salmonella in sprouts or spent irrigation water collected from sprouting of naturally contaminated seeds.
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http://dx.doi.org/10.4315/0362-028X.JFP-12-155 | DOI Listing |
Br Dent J
December 2024
School of Dentistry, International Medical University, Kuala Lumpur, Malaysia.
Objective(s) This questionnaire study aimed to analyse the irrigation knowledge and current trends in irrigation practice during endodontic treatment among general dental practitioners (GDPs) in Malaysia.Materials and methods A three-part, self-administered questionnaire with multiple-choice questions was distributed to dentists in Malaysia. The survey comprised three sections: demographic data; irrigant selection and protocol; and other irrigation practices.
View Article and Find Full Text PDFCochrane Database Syst Rev
October 2024
Florence Nightingale Faculty of Nursing, Midwifery and Palliative Care, King's College London, London, UK.
Background: People with central neurological disease or injury have a much higher risk of both faecal incontinence (FI) and constipation than the general population. There is often a fine line between the two symptoms, with management intended to ameliorate one risking precipitating the other. Bowel problems are observed to be the cause of much anxiety and may reduce quality of life in these people.
View Article and Find Full Text PDFSensors (Basel)
September 2024
Brazilian Agricultural Research Company (Embrapa) Soils, Rio de Janeiro 22460-000, RJ, Brazil.
The scientific field of precision agriculture employs increasingly innovative techniques to optimize inputs, maximize profitability, and reduce environmental impact. However, obtaining a high number of soil samples is challenging in order to make precision agriculture viable. There is a trade-off between the amount of data needed and the time and resources spent to obtain these data compared to the accuracy of the maps produced with more or fewer points.
View Article and Find Full Text PDFChemosphere
September 2024
Department of Science and Environmental Studies, The Education University of Hong Kong, Tai Po, Hong Kong Special Administrative Region, 999077, China. Electronic address:
Trop Anim Health Prod
August 2024
Department of Animal Science, Vale Acaraú State University, Sobral, Ceará, Brazil.
This study aimed to evaluate forage production and ingestive behavior of Morada Nova sheep in an intensive system with capim-tamani grass in both monoculture and silvopastoral irrigated systems in the Semi-arid region. Eighteen adult sheep, approximately 3 years old, with an average body weight of 26.8 ± 4.
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