Isolation and Identification of hydrocarbon degrading bacteria from Ennore creek.

Bioinformation

Department of Microbiology, SRM Arts & Science College, Kattangulathur - 603203, Kancheepuram Dist, Tamilnadu, India.

Published: February 2013

AI Article Synopsis

  • The discharge of petroleum products into marine environments poses significant hazards to ecosystems and life forms, necessitating effective remediation methods.
  • Bioremediation, which utilizes microorganisms for the clean-up of petroleum hydrocarbons, is highlighted as a cost-effective and non-invasive solution.
  • In this study, researchers identified and cultivated three efficient hydrocarbon-degrading bacterial isolates, with Pseudomonas aeruginosa I5 showing the highest degradation rate of 55% for crude oil.

Article Abstract

The widespread problem caused due to petroleum products, is their discharge and accidental spillage in marine environment proving to be hazardous to the surroundings as well as life forms. Thus remediation of these hydrocarbons by natural decontamination process is of utmost importance. Bioremediation is a non-invasive and cost effective technique for the clean-up of these petroleum hydrocarbons. In this study we have investigated the ability of microorganisms present in the sediment sample to degrade these hydrocarbons, crude oil in particular, so that contaminated soils and water can be treated using microbes. Sediments samples were collected once in a month for a period of twelve months from area surrounding Ennore creek and screened for hydrocarbon degrading bacteria. Of the 113 crude oil degrading isolates 15 isolates were selected and cultivated in BH media with 1% crude oil as a sole carbon and energy source. 3 efficient crude oil bacterial isolates Bacillus subtilis I1, Pseudomonas aeruginosa I5 and Pseudomonas putida I8 were identified both biochemically and phylogenetically. The quantitative analysis of biodegradation is carried out gravimetrically and highest degradation rate, 55% was recorded by Pseudomonas aeruginosa I5 isolate.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3569603PMC
http://dx.doi.org/10.6026/97320630009150DOI Listing

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