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Unlabelled: The aim of this in vitro study was to develop a replica technique aimed at reproducing an enamel surface exposed to erosive challenge. The replica technique was then used to compare the effect of treating enamel with different fluoride (F) solutions using an in vitro erosion model. Human molar teeth were split in two (total n=34 specimens), and half of each specimen was isolated with impression material. Part I: 10 specimens were exposed to either 0.18% or 1.8% citric acid, the impression material was removed and replicas were prepared. All specimens and replica models were analysed by profilometry and SEM. Part II: 24 specimens were treated with native 0.1 mol/L F-solutions (HF, SnF2, NaF and TiF4), the impression material was removed, and the specimens were then exposed to 1.8% citric acid. Replicas were prepared and analysed by profilometry and SEM. The median height difference between the F-treated areas and the non-treated areas for each F-solution was measured. SEM micrographs were analysed qualitatively blind bytwo of the authors.
Results: Part I--Replicas showed good topographical reproduction of the tooth specimens. Part II--HF had a significantly greater erosion-inhibiting effect than the other fluoride solutions at 0.1 mol/L F as shown by profilometry. SEM revealed a clearly defined border between F-treated and non-treated surfaces only for specimens treated with HF- and TiF solutions. In conclusion, the present replica technique was able to differentiate between the effect of treating enamel with different fluorides against dental erosion in vitro. The technique should be tested further for possible use in situ and in vivo.
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BMC Oral Health
December 2024
Dental Sciences Research Center, Department of Prosthodontics, School of Dentistry, Guilan University of Medical Sciences, Rasht, Iran.
Background: With the advances in the adhesive science, several conservative treatment options such as non-retentive overlays have been suggested for endodontically treated posterior teeth. However, information is scarce regarding the fracture strength and adaptation of non-retentive overlays compared with endocrowns, and the effect of material type in this respect. Thus, this study aimed to assess the fracture strength and internal and marginal adaptation of lithium disilicate (LDS) and hybrid ceramic endocrowns and non-retentive overlays for endodontically treated molar teeth.
View Article and Find Full Text PDFBiophys Chem
December 2024
School of Biotechnology, Jawaharlal Nehru University, New Delhi 110067, India. Electronic address:
Parkinson's disease (PD) is a neurodegenerative disorder involving the progressive loss of dopaminergic neurons in the substantia nigra pars compacta triggered by the accumulation of amyloid aggregates of α-synuclein protein. This study investigates the potential of Salvianolic Acid B (SalB), a water-soluble polyphenol derived from Salvia miltiorrhiza Bunge, in modulating the aggregation of the A53T mutant of α-synuclein (A53T Syn). This mutation is associated with rapid aggregation and a higher rate of protofibril formation in early-onset familial PD.
View Article and Find Full Text PDFSensors (Basel)
December 2024
Department of Mechanical and Aerospace Engineering, Missouri University of Science and Technology, Rolla, MO 65409, USA.
The combination of distributed digital factories (DFs) with sustainable practices has been proposed as a revolutionary technique in modern manufacturing. This review paper explores the convergence of DF with innovative sensor technology, concentrating on the role of Field Programmable Gate Arrays (FPGAs) in promoting this paradigm. A DF is defined as an integrated framework where digital twins (DTs), sensors, laser additive manufacturing (laser-AM), and subtractive manufacturing (SM) work in synchronization.
View Article and Find Full Text PDFMaterials (Basel)
November 2024
Department of Dental Materials Science, Academic Centre for Dentistry Amsterdam (ACTA), Universiteit van Amsterdam and Vrije Universiteit, 1081LA Amsterdam, The Netherlands.
This study aimed to evaluate the marginal and internal adaptation of CAD/CAM crowns milled using two different milling protocols (fine or extra-fine) within a 4-axis milling machine. The crowns were fabricated from lithium disilicate ceramic (IPS e.max CAD) and resin composite (Tetric CAD), assessing their fit in various regions.
View Article and Find Full Text PDFBiofabrication
December 2024
National Key Laboratory of Advanced Micro and Nano Manufacture Technology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China.
The specific spatiotemporal distribution of diverse components in tumor microenvironment plays a crucial role in the cancer progression.three-dimensional (3D) tumor models with polydimethylsiloxane (PDMS) based microfluidic platform have been applied as useful tool to conduct studies from cancer biology to drug screening. However, PDMS has not been welcomed as a standardized commercial application for preclinical screening due to inherent limitations in scale-up production and molecule absorption.
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