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The h-current in periglomerular dopaminergic neurons of the mouse olfactory bulb. | LitMetric

AI Article Synopsis

  • Researchers studied the hyperpolarization-activated cation current (I(h)) in rat dopaminergic neurons using advanced patch-clamp techniques and identified these neurons through a specific genetic marker.
  • The I(h) current activates around -82.7 mV and significantly contributes to the cell's resting potential, influencing its excitability but does not directly control the neurons' spontaneous firing.
  • Changes in intracellular cAMP levels affect I(h) kinetics, but neurotransmitters like dopamine and serotonin do not alter the I(h) amplitude, while noradrenaline reduces it, suggesting that I(h) affects how these neurons process sensory information without being part of the pacemaker activity.

Article Abstract

The properties of the hyperpolarization-activated cation current (I(h)) were investigated in rat periglomerular dopaminergic neurons using patch-clamp recordings in thin slices. A reliable identification of single dopaminergic neurons was made possible by use of a transgenic line of mice expressing eGFP under the tyrosine hydroxylase promoter. At 37 °C and minimizing the disturbance of the intracellular milieu with perforated patches, this current shows a midpoint of activation around -82.7 mV, with a significant level of opening already at rest, thereby giving a substantial contribution to the resting potential, and ultimately playing a relevant function in the control of the cell excitability. The blockage of I(h) has a profound influence on the spontaneous firing of these neurons, which result as strongly depressed. However the effect is not due to a direct role of the current in the pacemaker process, but to the I(h) influence on the resting membrane potential. I(h) kinetics is sensitive to the intracellular levels of cAMP, whose increase promotes a shift of the activation curve towards more positive potentials. The direct application of DA and 5-HT neurotransmitters, physiologically released onto bulbar dopaminergic neurons and known to act on metabotropic receptors coupled to the cAMP pathway, do not modifythe I(h) amplitude. On the contrary, noradrenaline almost halves the I(h) amplitude. Our data indicate that the HCN channels do not participate directly to the pacemaker activity of periglomerular dopaminergic neurons, but influence their resting membrane potential by controlling the excitability profile of these cells, and possibly affecting the processing of sensory information taking place at the entry of the bulbar circuitry.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3572079PMC
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0056571PLOS

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