AI Article Synopsis
- The study developed water-soluble CdTe quantum dots (QDs) that are combined with anti-HbA1c antibodies to create a specialized nanobioprobe for detecting HbA1c levels.
- A sandwich immunoassay was used, leveraging both fluorescence and electrochemical methods to accurately measure HbA1c concentrations, showing a strong linear relationship in results.
- Compared to traditional HPLC methods, the QD-based immunoassay demonstrated a high correlation in clinical sample analysis, indicating its potential for efficient and cost-effective HbA1c testing.
Article Abstract
Highly luminescent water soluble CdTe quantum dots (QDs) were synthesized and conjugated with anti-HbA1c antibody to generate specific nanobioprobe. A sandwich immunoassay model was employed using capture HbA1c antibody as a specific receptor molecule and the QD-labeled secondary antibody as a dual (fluorescence cum electrochemical) tracer to quantify the concentration of HbA1c. A linear increase in current was observed for HbA1c analytical standards with a R(2) value of 0.990 and coefficient of variance ~5%. A comparison between HPLC and dual immunoassay for clinical samples showed a correlation coefficient of 89% and 96% for fluorescence and electrochemical detection methods respectively. The QD-based immunoassay shows great promise for rapid reproducible and cost effective analysis of HbA1c in clinical samples.
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| http://dx.doi.org/10.1016/j.bios.2013.01.018 | DOI Listing |
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