AI Article Synopsis
- Researchers isolated a feruloyl esterase (FAE) gene from rumen microbes, cloned it in E. coli, and successfully expressed the enzyme called RuFae2.
- The RuFae2 enzyme effectively released ferulic acid from various plant materials, with the highest release from wheat-insoluble arabinoxylan and corn fiber.
- Combining RuFae2 with a GH10 endoxylanase (EX) significantly boosted ferulic acid release, particularly from wheat bran, while GH11 EX showed a considerably lesser effect.
Article Abstract
A feruloyl esterase (FAE) gene was isolated from a rumen microbial metagenome, cloned into E. coli, and expressed in active form. The enzyme (RuFae2) was identified as a type C feruloyl esterase. The RuFae2 alone released ferulic acid from rice bran, wheat bran, wheat-insoluble arabinoxylan, corn fiber, switchgrass, and corn bran in the order of decreasing activity. Using a saturating amount of RuFae2 for 100 mg substrate, a maximum of 18.7 and 80.0 μg FA was released from 100 mg corn fiber and wheat-insoluble arabinoxylan, respectively. Addition of GH10 endoxylanase (EX) synergistically increased the release of FA with the highest level of 6.7-fold for wheat bran. The synergistic effect of adding GH11 EX was significantly smaller with all the substrates tested. The difference in the effect of the two EXs was further analyzed by comparing the rate in the release of FA with increasing EX concentration using wheat-insoluble arabinoxylan as the substrate.
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| http://dx.doi.org/10.1007/s10295-013-1234-1 | DOI Listing |
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