Tetracycline resistance protein Tet(O), which protects the bacterial ribosome from binding the antibiotic tetracycline, is a translational GTPase with significant similarity in both sequence and structure to the elongation factor EF-G. Here, we present an atomic model of the Tet(O)-bound 70S ribosome based on our cryo-electron microscopic reconstruction at 9.6-Å resolution. This atomic model allowed us to identify the Tet(O)-ribosome binding sites, which involve three characteristic loops in domain 4 of Tet(O). Replacements of the three amino-acid tips of these loops by a single glycine residue result in loss of Tet(O)-mediated tetracycline resistance. On the basis of these findings, the mechanism of Tet(O)-mediated tetracycline resistance can be explained in molecular detail.
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http://dx.doi.org/10.1038/ncomms2470 | DOI Listing |
Vet Sci
December 2024
Faculty of Veterinary Science, University of Thessaly, 43100 Karditsa, Greece.
This study aimed to investigate the incidence of subclinical mastitis (SCM), the implicated pathogens, and their impact on milk quality in dairy sheep in Greece. Furthermore, we preliminarily evaluated infrared thermography and the application of AI tools for the early, non-invasive diagnosis of relevant cases. In total, 660 milk samples and over 2000 infrared thermography images were obtained from 330 phenotypically healthy ewes.
View Article and Find Full Text PDFVet Sci
December 2024
Department of Biomedical and Diagnostic Sciences, College of Veterinary Medicine, University of Tennessee, 2407 River Drive, Knoxville, TN 37996, USA.
This study evaluated the concentrations of tetracycline, erythromycin, and sulfonamide in beef, eggs, and honey products sold at grocery stores and whether standard cooking to 160 °F (71 °C) reduced the concentrations of antimicrobials spiked into the evaluated food products. In June 2021, the concentrations of selected antimicrobials in 65 samples (8 beef, 48 eggs, 9 honey samples) purchased from grocery stores within Knoxville, Tennessee, were measured using enzyme-linked immunosorbent assays. The Kruskal-Wallis test was used to assess the differences in the median tetracycline concentrations across food types, while the Mann-Whitney test was used to compare the erythromycin residue concentrations between beef and honey.
View Article and Find Full Text PDFMicrobiol Resour Announc
December 2024
Research Department for Limnology, Mondsee, Universität Innsbruck, Mondsee, Austria.
Mapping transcription start sites and determining their activity remain a challenging task even for well-studied organisms. Here, we present Cappable-seq RNA sequencing data of K-12 MG1655 after treatment with three antibiotics with various spectra of action that may expand the range of mapped transcription start sites for this model organism.
View Article and Find Full Text PDFInt J Antimicrob Agents
December 2024
Animal and Human Health Department, International Livestock Research Institute, Nairobi, Kenya.
Peri-urban environments, characterized by dense human populations, cohabiting livestock, and complex food systems, serve as hotspots for food contamination and infectious diseases. Children aged 6-24 months are particularly vulnerable as they often encounter contaminated food and water, increasing their risk of food-borne disease, with diarrhea being a common symptom. We investigated the prevalence of antimicrobial resistance (AMR) in pathogenic Escherichia coli from 6-24 months-old children, their food, and cohabiting livestock, in Dagoretti South subcounty in Nairobi, Kenya.
View Article and Find Full Text PDFPoult Sci
December 2024
Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture and Rural Affairs), Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, China. Electronic address:
Pigeon has become popular as a food recently, due to their highly nutritive valuE. colibacillosis, which is caused by pathogenic E. coli, is one of the most important bacterial diseases in pigeon breeding.
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