The human pathogen Giardia lamblia is an anaerobic protozoan parasite that causes giardiasis, one of the most common diarrheal diseases worldwide. Although several drugs are available for the treatment of giardisis, resistance to these drugs has been reported and is likely to increase. The Giardia carbamate kinase (glCK) plays an essential role in Giardia metabolism and has no homologs in humans, making it an attractive candidate for anti-Giardia drug development. We have developed a luminescent enzyme coupled assay to measure the activity of glCK by quantitating the amount of ATP produced by the enzyme. This assay is homogeneous and has been miniaturized into a 1536-well plate format. A pilot screen against 4,096 known compounds using this assay yielded a signal-to-basal ratio of 11.5 fold and Z' factor of 0.8 with a hit rate of 0.9 % of inhibitors of glCK. Therefore, this Giardia lamblia carbamate kinase assay is useful for high throughput screening of large compound collection for identification of the inhibitors for drug development.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3565245 | PMC |
| http://dx.doi.org/10.2174/1875397301206010093 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Prevalence and factors associated with intestinal parasitosis in children from an urban slum in Brazil: a cross-sectional study.
Rev Paul Pediatr
January 2025
Universidade Federal de Uberlândia, Faculdade de Odontologia, Uberlândia, MG, Brazil.
Objective: This study aimed to estimate the prevalence and investigate the factors associated with intestinal parasitic diseases in children from an urban slum in Brazil.
Methods: A cross-sectional study was conducted in children living in SEWA community, an urban slum located in Araguari, Minas Gerais, Brazil. The prevalence of intestinal parasitosis was determined via stool parasitological examination by spontaneous sedimentation.
Determinants of Anemia in Schoolchildren in the Highland Bolivia.
Microorganisms
December 2024
Unidad de Inmunología Parasitaria, Facultad de Medicina, Universidad Mayor de San Andrés, La Paz 10077, Bolivia.
Anemia is a health problem of concern among schoolchildren in underprivileged rural regions, where recurrent parasitic infections are common. A cross-sectional study was conducted in 229 schoolchildren in rural highland Bolivia in the department of La Paz, an area with a high prevalence of protozoan and helminth infections, to determine the types and mechanisms of anemia. A substantial proportion of children (40.
View Article and Find Full Text PDFOccurrence and Molecular Characterization of Cryptosporidium spp. and Giardia duodenalis in Water Buffaloes (Bubalis bubalis) From Türkiye.
Acta Parasitol
January 2025
Department of Parasitology, Faculty of Veterinary Medicine, Sivas Cumhuriyet University, 58140, Sivas, Türkiye.
Purpose: Cryptosporidium spp. and Giardia duodenalis are zoonotic protozoan parasites that are widely seen in domestic and wild animals worldwide. While these pathogens, which affect the digestive system of the hosts, cause high economic losses in animal breeding, they are also considered an important public health problem.
View Article and Find Full Text PDFPrevalence of Intestinal Parasitic Infections and Associated Factors Among Food Handlers in East Africa: A Systematic Review and Meta-analysis.
Acta Parasitol
January 2025
Department of Medical Laboratory Science, College of Health Sciences, Woldia University, P.O. box 400, Woldia, Ethiopia.
Background: Intestinal parasitic infections are a significant public health concern, especially among food handlers, who can transmit these infections to the public through food preparation and handling. This systematic review and meta-analysis aimed to determine the pooled prevalence and associated factors of intestinal parasitic infections among food handlers in the East African region.
Methods: A systematic review and meta-analysis on intestinal parasitic infections among food handlers involved a comprehensive search across various databases, including Scopus, PubMed, ScienceDirect, Google Scholar, and the institution's library registers.
Prevalence of Intestinal Protozoan Parasites among Children Attending the Hevi Pediatric Hospital in Duhok Province, Kurdistan Region, Iraq.
Arch Razi Inst
June 2024
Department of Medical Laboratory Sciences, College of Health Sciences, University of Duhok, Iraq.
Article Synopsis
- The study focused on the prevalence of intestinal protozoan infections among children in Duhok Province, Iraq, from October 2022 to May 2023, involving 740 pediatric patients.
- A total of 205 children (27.7%) tested positive for infections, with the highest rates found in the 5-9 age group, and significant variations in prevalence noted among different age categories.
- The findings indicate that amoebiasis and cryptosporidiosis are more common than previously reported in Iraq, while giardiasis occurred at lower rates, highlighting the need for improved public health measures.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!