Artificial activators designed using transcription activator-like effector (TALE) technology have broad utility, but previous studies suggest that these monomeric proteins often exhibit low activities. Here we demonstrate that TALE activators can robustly function individually or in synergistic combinations to increase expression of endogenous human genes over wide dynamic ranges. These findings will encourage applications of TALE activators for research and therapy, and guide design of monomeric TALE-based fusion proteins.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584229 | PMC |
| http://dx.doi.org/10.1038/nmeth.2366 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A tale of two ages: fluid reasoning as a predictor of working memory training efficacy in middle-aged and older adults.
Neuropsychol Dev Cogn B Aging Neuropsychol Cogn
January 2025
Department of Psychology, Faculty of Humanities and Social Sciences, University of Zagreb, Zagreb, Croatia.
Research on working memory (WM) training reveals significant variability in training effects, indicating that pretraining cognitive abilities might account for these differences. However, consensus on whether higher (magnification account) or lower (compensation account) pretraining abilities predict greater training effects remains elusive. Our study aimed to clarify the role of fluid reasoning in predicting training performance (i.
View Article and Find Full Text PDFTwo TAL Effectors of Xanthomonas citri pv. malvacearum Induce Water Soaking by Activating GhSWEET14 Genes in Cotton.
Mol Plant Pathol
January 2025
Shanghai Collaborative Innovation Center of Agri-Seeds/State Key Laboratory of Microbial Metabolism, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
Bacterial blight of cotton (BBC) caused by Xanthomonas citri pv. malvacearum (Xcm) is an important and destructive disease affecting cotton plants. Transcription activator-like effectors (TALEs) released by the pathogen regulate cotton resistance to the susceptibility.
View Article and Find Full Text PDFActivation of three targets by a TAL effector confers susceptibility to bacterial blight of cotton.
Nat Commun
January 2025
Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI, 48109, USA.
Bacterial transcription activator-like effectors (TALEs) promote pathogenicity by activating host susceptibility (S) genes. To understand the pathogenicity and host adaptation of Xanthomonas citri pv. malvacearum (Xcm), we assemble the genome and the TALE repertoire of three recent Xcm Texas isolates.
View Article and Find Full Text PDFThe Effects of Osteopathic Manipulative Treatment on Arrhythmias: A Double-blind Randomized Controlled Trial in Patients with Cardiac Implantable Electronic Devices.
J Innov Card Rhythm Manag
December 2024
Department of Clinical Specialties, New York Institute of Technology College of Osteopathic Medicine, Old Westbury, NY, USA.
This double-blind randomized controlled trial investigated the effects of osteopathic manipulative treatment (OMT) on cardiac arrhythmias in patients with cardiac implantable electronic devices (CIEDs). Participants (n = 41) with CIEDs were randomly assigned to either the OMT group or the control group (light touch/sham) and received a one-time intervention. No significant change in arrhythmia burden was found in the 1 month following intervention ( = .
View Article and Find Full Text PDFThe mysterious case of missing lymphocytes: a cautionary tale of inter-institutional variability in outcomes of lung dissociation protocols.
Am J Physiol Lung Cell Mol Physiol
January 2025
Dept. of Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA.
Rigor and reproducibility are vital to scientific advancement. It is unclear whether a protocol optimized for tissue dissociation in one institution performs well universally. Here, we share our brand-new lab's experience with inter-institutional variability that led to the discovery that a protocol optimized for murine lung dissociation at Boston University (BU) fails to reproduce similar CD4 T cell, CD8 T cell, and B cell outcomes at the University of Michigan at Ann Arbor (U-M).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!