Conventional methods for the identification of human-pathogenic aerococci to the species level are not reliable. We show that matrix-assisted laser desorption ionization-time of flight mass spectrometry correctly identifies aerococci to the species level and that it can be used to identify aerococci with high specificity in the diagnostic clinical microbiology laboratory.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3666765PMC
http://dx.doi.org/10.1128/JCM.02637-12DOI Listing

Publication Analysis

Top Keywords

matrix-assisted laser
8
laser desorption
8
desorption ionization-time
8
ionization-time flight
8
flight mass
8
mass spectrometry
8
aerococci species
8
species level
8
spectrometry sensitive
4
sensitive specific
4

Similar Publications

Cardiovascular disease (CVD) is the leading cause of death in the United States. Damage in the cardiovascular system can be due to environmental exposure, trauma, drug toxicity, or numerous other factors. As a result, cardiac tissue and vasculature undergo structural changes and display diminished function.

View Article and Find Full Text PDF

is an anaerobic, gram-negative bacillus commonly associated with acute appendicitis. However, bacteremia is exceedingly rare. Herein, we report a case of bacteremia associated with a urethrocutaneous fistula and a subcutaneous abscess in the left inguinal region.

View Article and Find Full Text PDF

The expansion of the seafood market has led to an increased probability of food fraud. The development of rapid and reliable traceability methods for aquatic food products is of utmost importance. In this study, direct analysis and identification of the intestinal microbiota of aquatic foods were conducted.

View Article and Find Full Text PDF

Analysis of the spatial distribution of metabolites in Aloe vera leaves by mass spectrometry imaging and UHPLC-UHRMS.

Sci Rep

January 2025

Department of Polymers and Biopolymers, Faculty of Chemistry, Rzeszów University of Technology, 6 Powstańców Warszawy Ave., Rzeszów, 35-959, Poland.

This study presents an investigation of the chemical composition of Aloe vera leaf tissue with a focus on the spatial distribution of compounds. The composition was studied using two mass spectrometry imaging techniques: silver-109 nanoparticles assisted laser desorption/ionization mass spectrometry imaging (AgNPs-LDI-MSI) and laser ablation-remote atmospheric pressure photoionization/chemical ionization mass spectrometry imaging (LARAPPI/CI-MSI) and the identification was aided by ultra-high-performance liquid chromatography and ultra-high-resolution mass spectrometry (UHPLC-UHRMS) analysis. The results showed an abundance of phenolic compounds with antioxidant, antimicrobial, and anti-inflammatory properties, making it a beneficial food additive and food packaging material.

View Article and Find Full Text PDF

Use of analytical strategies to understand spatial chemical variation in bacterial surface communities.

J Bacteriol

January 2025

Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, Notre Dame, Indiana, USA.

Not only do surface-growing microbes such as biofilms display specific traits compared to planktonic cells, but also they display many heterogeneous behaviors over many spatial and temporal contexts. While the application of molecular genetics tools to extract or visualize gene expression or regulatory function data is now common in studying surface growth, the use of analytical chemistry tools to visualize the spatiotemporal distribution of chemical products synthesized by these surface microbes is less common. Here, we review chemical imaging tools that have been used to inform our understanding of surface-growing microbes.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!