[Influence of acupuncture or thermal acupuncture stimulation at "Zusanli" (ST 36) on thoracic duct lymph volume and the relevant chemical substances in normal rats].

Objective: To observe the changes of thoracic duct lymph volume and the contents of histamine (HA), 5-hydroxytryptamine (5-HT), etc. in the lymph after acupuncture or thermal acupuncture interventions, in order to investigate the effect of lymphatic system in transmitting acupuncture and moxibustion signals.

Methods: A total of 45 male Wistar rats were randomly divided into control group, acupuncture group, and thermal acupuncture (acupuncture with the needle warmed by burning moxa) group (n = 15/group). The rat thoracic duct lymphatic fistula model was replicated. Acupuncture or thermal acupuncture was applied to "Zusanli" (ST 36), and the changes of thoracic duct lymph volume and the concentrations of HA, 5-HT in the lymph were observed.

Results: Compared with the control group, the thoracic duct lymph volume in acupuncture group and thermal acupuncture group were obviously increased (P < 0.05), while the concentrations of lymph HA and 5-HT in both acupuncture and thermal acupuncture groups had no significant changes (P > 0.05).

Conclusion: Both acupuncture and thermal acupuncture interventions can increase the thoracic duct lymph volume, but have no effects on lymph HA and 5-HT contents in normal rats.