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We present a case of mixed microbial keratitis in an otherwise healthy contact lens wearer. The microbes detected on microscopy included , , , and . and are well-recognised corneal pathogens, although is uncommon, and is extremely rare.
View Article and Find Full Text PDFInvestigating the validity of mCIM and sCIM phenotypic methods in screening Pseudomonas aeruginosa isolates producing IMP, VIM, and NDM metallo-beta-lactamases isolated from burn wounds.
J Antibiot (Tokyo)
January 2025
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Metallo-beta-lactamase-producing Pseudomonas aeruginosa (P. aeruginosa) is a major pathogen in burn wounds, often exhibiting high levels of antibiotic resistance, which complicates treatment strategies. This study deals with the validity of the modified Carbapenem Inactivation Method (mCIM) and the simplified Carbapenem Inactivation Method (sCIM) phenotypic tests for screening metallo-beta-lactamase (MBL) production by P.
View Article and Find Full Text PDFDistribution of the four type VI secretion systems in Pseudomonas aeruginosa and classification of their core and accessory effectors.
Nat Commun
January 2025
Institute for Experimental Medicine, Kiel University, Kiel, Germany.
Bacterial type VI secretion systems (T6SSs) are puncturing molecular machines that transport effector proteins to kill microbes, manipulate eukaryotic cells, or facilitate nutrient uptake. How and why T6SS machines and effectors differ within a species is not fully understood. Here, we applied molecular population genetics to the T6SSs in a global population of the opportunistic pathogen Pseudomonas aeruginosa.
View Article and Find Full Text PDFAntimicrobial proteins from oyster hemolymph improve the efficacy of conventional antibiotics.
PLoS One
January 2025
National Marine Science Centre, Southern Cross University, Coffs Harbour, NSW, Australia.
Discovering new antibiotics and increasing the efficacy of existing antibiotics are priorities to address antimicrobial resistance. Antimicrobial proteins and peptides (AMPPs) are considered among the most promising antibiotic alternatives and complementary therapies. Here, we build upon previous work investigating the antibacterial activity of a semi-purified hemolymph protein extract (HPE) of the Australian oyster Saccostrea glomerata.
View Article and Find Full Text PDFPrevalence of Plasmid-Mediated Fluoroquinolone Resistance Genes in Pseudomonas aeruginosa Among Patients at Aleppo University Hospital, Syria.
J Clin Lab Anal
January 2025
Department of Microbiology, Faculty of Sciences, University of Aleppo, Aleppo, Syria.
Background: Pseudomonas aeruginosa is a significant opportunistic pathogen, especially in hospital-acquired infections, with plasmid-mediated fluoroquinolone resistance posing a major healthcare threat. This research aimed to isolate fluoroquinolone-resistant P. aeruginosa from patients at Aleppo University Hospital, assess the prevalence of fluoroquinolone resistance, confirm molecular identity, identify plasmid-associated resistance genes, and investigate virulence factors.
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