Low concentrations of Campylobacter jejuni cells in environmental samples make them difficult to study with conventional culture methods. Here, we show that enrichment by amoeba cocultures works well with low-concentration samples and that this method can be combined with molecular techniques without loss of genetic specificity.
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http://dx.doi.org/10.1128/AEM.02918-12 | DOI Listing |
Proc Natl Acad Sci U S A
January 2025
Chinese Academy of Sciences Key Laboratory of Tropical Marine Bio Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, Innovation Academy of South China Sea Ecology and Environmental Engineering, Guangdong Provincial Observation and Research Station for Coastal Upwelling Ecosystem, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 511458, China.
Rotation of the bacterial flagellum, the first identified biological rotary machine, is driven by its stator units. Knowledge gained about the function of stator units has increasingly led to studies of rotary complexes in different cellular pathways. Here, we report that a tetrameric PilZ family protein, FlgX, is a structural component underneath the stator units in the flagellar motor of .
View Article and Find Full Text PDFMicrobiol Resour Announc
January 2025
Egg and Poultry Production Safety Research Unit, USDA Agricultural Research Service, Athens, Georgia, USA.
We present the draft genomic sequences of 65 isolates of spp. (38 and 27 ) isolated from table egg-layer chickens, which are not generally associated with human transmission. Up to this time, there are no publicly available genomic sequences of isolated from laying hens.
View Article and Find Full Text PDFPoult Sci
January 2025
Prestage Department of Poultry Science, College of Agriculture and Life Sciences, North Carolina State University, Raleigh, NC 27695-7608. Electronic address:
Campylobacter infections are a prevalent cause of diarrheal disease in humans and are the most significant zoonotic pathogens worldwide. Human campylobacteriosis is generally via ingestion of contaminated poultry products. However, based on recent studies chicken egg yolk antibody (IgY) powder has great potential to reduce the cecum load of Campylobacter jejuni (C.
View Article and Find Full Text PDFCan Vet J
January 2025
Department of Clinical Studies (Kritikos, Monteith, Bateman) and Department of Pathobiology (Weese), Ontario Veterinary College, University of Guelph, 50 Stone Road East, Guelph, Ontario N1G 2W1.
Objective: To determine the prevalence of fecal microorganisms and parasites in a population of sheltered cats, and to identify specific animal factors associated with infection.
Animals: A total of 79 sheltered cats and kittens in Guelph, Ontario.
Procedure: A fecal sample was collected from each animal upon shelter entry.
mSphere
January 2025
Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA.
Post-transcriptional gene regulation by non-coding small RNAs (sRNAs) is critical for colonization and survival of enteric pathogens, including the zoonotic pathogen . In this study, we utilized IA3902 (a representative isolate of the sheep abortion clone) and W7 (a highly motile variant of NCTC 11168, a human gastroenteritis strain) to further investigate regulation by sRNA CjNC110. Both motility and autoagglutination ability were confirmed to be phenotypes of conserved regulation by CjNC110.
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