A dinuclear ruthenium(II) complex linked via a reducible azo group [Ru(bpy)2(azobpy)Ru(bpy)2]Cl4 (Ru2azo, bpy=2,2'-bipyridine, azobpy=4,4″-azobis (2,2'-bipyridine)) was adopted as a probe for thiols. Results showed that Ru2azo could selectively and effectively react with biological thiols (such as cysteine, homocysteine and glutathione) with a 10(-7)M detection limit. After it reacted with thiols, the original gray color of Ru2azo solution immediately turned yellow and the luminescence significantly enhanced, showing "naked-eye" colorimetric and "off-on" luminescent dual-signaling response for thiols. Mechanism studies demonstrated that Ru2azo reacted with thiols undergoing a two-electron transfer process, forming the azo(2-) anion product.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jinorgbio.2012.12.019 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Target Deoxyribonucleic Acid-Recycled Lighting-Up Amplifiable Ratiometric Fluorescence Biosensing of Bicolor Silver Nanoclusters Hosted in a Switchable Deoxyribonucleic Acid Construct.
Anal Chem
May 2022
Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Ratiometric assays of label-free dual-signaling reporters with enzyme-free amplification are intriguing yet challenging. Herein, yellow- and red-silver nanocluster (H-AgNC and H-AgNC) acting as bicolor ratiometric emitters are guided to site-specifically cluster in two template signaling hairpins (H and H), respectively, and originally, both of them are almost non-fluorescent. The predesigned complement tethered in H is recognizable to a DNA trigger () related to SARS-CoV-2.
View Article and Find Full Text PDFHighly Stable Upconverting Nanocrystal-Polydiacetylenes Nanoplates for Orthogonal Dual Signaling-Based Detection of Cyanide.
ACS Appl Mater Interfaces
January 2020
School of Energy and Chemical Engineering , Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919 , Republic of Korea.
Although the unique optical signaling properties of polydiacetylene (PDA) have been exploited in diverse bio-chemosensors, the practical application of most PDA sensor systems is limited by their instability in harsh environments and fluorescence signal weakness. Herein, a universal design principle for a highly stable PDA sensor system with a practical dual signaling capability is developed to detect cyanide (CN) ions, which are commonly found in drinking water. Effective metal intercalation and enhanced hydrophobic intermolecular interactions between PDA-metal supramolecules are used to construct highly stacked PDA-metal nanoplates that feature unusual optical stability upon exposure to strong acids, bases, organic solvents, and thermal/mechanical stresses, and can selectively detect CN anions, concomitantly undergoing a specific supramolecular structure change.
View Article and Find Full Text PDFHighly selective ratiometric electrogenerated chemiluminescence assay of DNA methyltransferase activity via polyaniline and anti-fouling peptide modified electrode.
Biosens Bioelectron
October 2019
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, Shandong Key Laboratory of Biochemical Analysis, Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, PR China. Electronic address:
In this work, an antifouling electrochemiluminescent (ECL) ratiometric biosensor is designed for the accurate, selective and sensitive detection of DNA methyltransferase (MTase) activity based on a dual-signaling strategy. Briefly, an ITO electrode is used to construct the anti-fouling interfaces with the modification of polyaniline (PANI), AuNPs and peptide. Hairpin DNA molecules containing the symmetric sequence of 5'-CATC-3' are attached onto the modified ITO electrode and the ds-DNA can be cut off in the presence of Dam MTase and DpnI.
View Article and Find Full Text PDFFunctionalized gold and persistent luminescence nanoparticle-based ratiometric absorption and TR-FRET nanoplatform for high-throughput sequential detection of l-cysteine and insulin.
Nanoscale
August 2018
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
In vitro diagnostic is a crucial component of healthcare systems for early diagnosis of diseases and follow-up therapy, which generally makes clinical diagnosis faster, easier, and painless for patients. Herein, we report a dual-signaling nanoplatform for ratiometric absorption and time-resolved fluorescence resonance energy transfer based on l-cysteine-mediated aggregated gold nanoparticles and long afterglow nature of persistent luminescence nanoparticles. With this nanoplatform, we have achieved high-throughput sequential detection of l-cysteine and insulin in human serum without matrix interference.
View Article and Find Full Text PDFFrom Electrochemistry to Electroluminescence: Development and Application in a Ratiometric Aptasensor for Aflatoxin B1.
Anal Chem
July 2017
State Key Laboratory of Agricultural Microbiology, College of Food Science and Technology, College of Science, Huazhong Agricultural University, Wuhan 430070, People's Republic of China.
Aflatoxin B1 (AFB1), one of the most toxic chemical carcinogens, has been widely studied. It remains challenging to develop simple, accurate, and sensitive analytical methods for the detection of AFB1 in food matrixes. In this work, on the basis of a dual-signaling strategy, a ratiometric aptasensor was designed and verified for the accurate and sensitive detection of AFB1.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!