AI Article Synopsis
- The study investigates the role of the protein Sema6A in the migration of oligodendrocyte precursor cells (OPCs) in the brain, specifically in the context of Plexin-A4 knockout mice.
- Research revealed that reducing Sema6A levels led to decreased migration of OPCs in lab settings.
- Findings suggest that Sema6A affects OPC migration by interacting with the Plexin-A4 receptor in neighboring cells, which is important for understanding cell movement in brain development.
Article Abstract
Our previous study has confirmed that the distribution of oligodendrocyte precursor cells (OPCs) is disturbed in the embryonic cerebral cortex of Plexin-A4 knockout mice, and that Sema6A is expressed in OPCs in the region. The present study examined whether Sema6A expressed in OPCs is involved in their own migration, and used a clonal FBD-102b line as OPCs model. In an in vitro migration assay, Sema6A knockdown repressed the migration of FBD-102b cells. Additionally, in co-culture, 3T3 cells ectopically expressing Plexin-A4 were segregated from 3T3 cells ectopically expressing Sema6A. When FBD-102b cells were seeded in a spot and exposed to a gradient of both Sema3A and Sema6A, dispersion of FBD-102b cells was suppressed, and Plexin-A4 knockdown in FBD-102b cells attenuated the suppressive effect of the Semaphorins. These results indicate that Sema6A expressed in OPCs is involved in their autonomous migration through ligand-receptor interaction with Plexin-A4 expressed in surrounding cells.
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| http://dx.doi.org/10.1016/j.neulet.2013.01.026 | DOI Listing |
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