We previously identified a polyketide synthase gene cluster, aur1, responsible for the production of the angucycline antibiotic auricin in Streptomyces aureofaciens CCM 3239. A sequence analysis of the aur1 flanking regions revealed the presence of several genes encoding proteins homologous to those for Streptomyces linear plasmid replication, partitioning and telomere-binding. Pulse-field gel electrophoresis detected the single, 240-kb linear plasmid, pSA3239, in S. aureofaciens CCM3239. The presence of the auricin cluster in pSA3239 was confirmed by several approaches. In addition to aur1, pSA3239 also carries a large number of regulatory genes, and two gene clusters involved in the production of secondary metabolites: the aur2 cluster for an unknown secondary metabolite and the bpsA cluster for the blue pigment indigoidine.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/1574-6968.12095 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Nicking Activity of M13 Bacteriophage Protein 2.
Int J Mol Sci
January 2025
Department of Biomedical Engineering, Faculty of Engineering and Natural Sciences, Acibadem Mehmet Ali Aydinlar University, 34752 Istanbul, Türkiye.
Gene II Protein (Gp2/P2) is a nicking enzyme of the M13 bacteriophage that plays a role in the DNA replication of the viral genome. P2 recognizes a specific sequence at the f1 replication origin and nicks one of the strands and starts replication. This study was conducted to address the limitations of previous experiments, improve methodologies, and precisely determine the biochemical activity conditions of the P2 enzyme in vitro.
View Article and Find Full Text PDFUnlabelled: is an emergent fungal pathogen of significant interest for molecular research because of its unique nosocomial persistence, high stress tolerance and common multidrug resistance. To investigate the molecular mechanisms of these or other phenotypes, a handful of CRISPR-Cas9 based allele editing tools have been optimized for . Nonetheless, allele editing in this species remains a significant challenge, and different systems have different advantages and disadvantages.
View Article and Find Full Text PDFComplete genome sequences of two actinomycetes containing abyssomicin-like gene clusters: DSM 45791 and sp. NL15-2K.
Microbiol Resour Announc
January 2025
School of Natural and Environmental Sciences, Newcastle University, Newcastle upon Tyne, United Kingdom.
Here, we report the resequencing, assembly, and annotation of two actinomycete genomes containing abyssomicin gene clusters. DSM 45791 with a circular chromosome of 11,681,598 bp and 4 circular plasmids (14,175-207,548 bp) and sp. NL15-2K with a 12,368,159 bp linear genome and circular plasmid (11,584 bp).
View Article and Find Full Text PDFWhole-genome sequences of six strains obtained via PacBio sequencing.
Microbiol Resour Announc
January 2025
Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, Michigan, USA.
Provided are whole-genome sequences of six strains that had been earlier isolated from louse-borne relapsing fever patients. The sequences of each genome presented here included one linear chromosome and 5 linear plasmids, whose average size was 1,284,895 bp with the mean GC content being 27.5%.
View Article and Find Full Text PDFBlue-LED assisted Photodegradation kinetics of rhodamine-6G dye, enhanced anticancer activity and cleavage of plasmids using Au-ZnO nanocomposite.
Heliyon
January 2025
School of Life Sciences, Department of Biochemistry, Molecular Oncology Laboratory, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India.
The plasmonic metal doping on the UV-active metal oxide nanoparticle turns the resultant plasmonic metal-metal oxide (PMMO) into visible light active and upon exogenous illumination the photogenerated energetic charge carriers and the generated reactive oxygen species (ROS, e.g. ·OH and O ) authoritatively enhances its biological and catalytic activity.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!