Background: Cytologists are often under time pressure due to a constant need and a demand for quick despatch of reports, which calls for an early assessment of sample adequacy.
Objective: To study whether unstained smears are effective in evaluation of sample adequacy of cytology aspirates.
Materials And Methods: The study had 3 groups. Assessment of sample adequacy of cytology aspirates was done on unstained smears in Group I (200 cases), Group II (100 cases out of Group I) and Group III ( 80 cases out of Group II ) by a Professor, Assistant Professor and a junior resident respectively. The results were compared with assessment of adequacy on stained smears and statistical analysis was done.
Results: Evaluation of adequacy of cytology aspirates by examination of unstained and stained smears by 3 observers of varied experience revealed no significant difference between the observers and between the stained and unstained smears (p<0.001).
Conclusion: Unstained smear study offered advantages over the stained smears in the evaluation of sample adequacy. Hence, it maybe recommended as a routine practice in cytology clinics.
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http://dx.doi.org/10.7860/JCDR/2012/4527.2642 | DOI Listing |
medRxiv
November 2024
Chan Zuckerberg Biohub SF, 499 Illinois Street, San Francisco, CA.
Malaria diagnostic testing in high transmission settings remains a burden on healthcare systems. Here we present Remoscope, a portable automated imaging cytometer that scans fresh, unstained whole blood using a custom neural network on low-cost hardware. By screening up to two million red blood cells, Remoscope performs label-free quantitative stage-specific detection of () in 1-12 minutes without sample fixation, staining, or slide scanning.
View Article and Find Full Text PDFVet Clin Pathol
June 2024
Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, Michigan, USA.
Background: Lipofuscin-like cytoplasmic inclusions have been reported in human blood neutrophils and monocytes but have not been described in dogs. In people, these "green granules of death" have been associated with moderate to severe hepatocellular injury and high mortality.
Objectives: To describe clinicopathologic abnormalities, diagnoses, and outcomes of dogs with greenish inclusions in blood neutrophils or monocytes, and to determine if the inclusions have features of lipofuscin.
Diagn Cytopathol
June 2024
Department of Pathology, University Hospital of Salerno, Salerno, Italy.
Cancer Cytopathol
May 2024
Department of Pathology, Stanford University School of Medicine, Stanford, California, USA.
Background: Circulating tumor cells (CTCs) shed into blood provide prognostic and/or predictive information. Previously, the authors established an assay to detect carcinoma cells from pleural fluid, termed effusion tumor cells (ETCs), by employing an immunofluorescence-based CTC-identification platform (RareCyte) on air-dried unstained ThinPrep (TP) slides. To facilitate clinical integration, they evaluated different slide processing and storage conditions, hypothesizing that alternative comparable conditions for ETC detection exist.
View Article and Find Full Text PDFJ Med Cases
January 2024
Department of Diagnostics Units, Fundacion Arturo Lopez Perez, Providencia, Chile.
Plasma cell leukemia (PCL) is a clinically aggressive variant of multiple myeloma, characterized by a high burden of circulating plasma cells, necessitating swift and accurate diagnosis due to its poor prognosis. The conventional diagnostic criteria, including the recent recommendation by the International Myeloma Working Group (IMWG) of > 5% circulating plasma cells as positive, have evolved over time. In this context, we present a detailed case report that underscores the pivotal role of the ADVIA 2120 automated hematology counter in detecting plasma cells through cytogram analysis, along with the significance of routine peripheral blood smear analysis and the utility of a large unstained cells (LUCs) threshold of > 4.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!