For complete and conclusive DNA profiling a sufficient amount of DNA must be available. For that, biological traces are recovered from crime scenes using special trace recovery material. The current method to collect biological traces for DNA analysis is to wipe them off with cotton swabs. However, for a few years the use of adhesive tapes has also been described for the recovery of evidence. In 2009, an adhesive tape was launched which was specially developed for the collection of biological traces. This product called Scenesafe FAST Tape (SSF) was investigated in this work to give recommendations for its use in evidence recovery. The results of this work show that the DNA can be extracted from the SSF very well. However, the tapes seem less suitable for direct use at the crime scene, as they are not flexible enough for adaptation to different crime scene conditions and the risk of contamination is higher than when collecting evidence with cotton swabs. Neither SSF nor cotton swabs are optimal for all requirements. The best method to recover biological evidence is still dependent on the surface material and the circumstances at the crime scene.
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Sci Rep
January 2025
Laboratory of Pharmacology and Toxicology of Natural Products, Biological Sciences Institute, Federal University of Pará (UFPA), UFPA Campus Belém, Rua Augusto Corrêa No 01 Guamá, Belém, PA, CEP: 66075 - 110, Brazil.
The present study aimed to characterize the behavior and evaluate the electromyographic (EMG), electrocardiographic (ECG), and respiratory responses of Nile tilapia (Oreochromis niloticus) when exposed to different concentrations of Camphor (CPR) as a potential anaesthetic in immersion and recovery baths. The goal was to determine the impact of CPR on muscle, cardiac, and respiratory functions, and assess its suitability as an anesthetic for tilapia. Therefore, juvenile fish (38.
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School of Pharmaceutical Sciences, University of Geneva, Geneva, Switzerland.
The isolation of pure compounds from complex extracts is a crucial step in natural products (NPs) research. Historically, this process has been recognized to be slow and laborious. However, significant advancements have been made in isolation methods.
View Article and Find Full Text PDFMethods Mol Biol
January 2025
Natural Products Lab, Institute of Biology, Leiden University, Leiden, The Netherlands.
This introductory chapter traces the evolution of (bio)chemical assays, emphasizing the critical role of robust protocols in ensuring reproducibility-a fundamental aspect of scientific research. With the advent of systems biology, the need for standardized methods has intensified, particularly for integrating vast datasets in open-access formats. The historical progression from basic plant morphology to advanced chromatographic and spectroscopic techniques in phytochemistry highlights the necessity for precise, reproducible protocols.
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Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Carbon Neutrality Interdisciplinary Science Centre, College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China.
Data-driven approaches (e.g., machine learning) are increasingly used to replace or assist laboratory studies in the study of emerging contaminants (ECs).
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February 2025
College of Agronomy, Hunan Agricultural University, Changsha 410128, China.
Seed color is a critical quality trait in numerous plant species. In oilseed crops, including rapeseed and mustard, yellow seeds are distinguished by their significantly higher oil content and faster germination rates compared to black or brown counterparts. Despite the agronomic significance of the yellow seeds being a prime breeding target, the mechanisms underlying elevated oil content remain obscure.
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