Objective: To study CXCR3 and CCR5 chemokine receptor expression in spleens of patients with primary immune thrombocytopenia (ITP) and its clinical significance.
Methods: The splenectomy specimens from 10 ITP patients (ITP group) and 8 patients with traumatic splenic rupture (normal control group) were studied. Immunohistochemistry (IHC) was used to study the positive rate of CXCR3 and CCR5. Western blot was performed to detect CXCR3 and CCR5 protein expression, while real-time polymerase chain reaction (RT-PCR) was conducted to analyze their mRNA expression.
Results: The positive rate of CXCR3 and CCR5 were both higher in ITP group (90% and 100%, respectively) than those in control group (75% and 87.5%, respectively)(P < 0.05). The differences were statistically significant (P < 0.05). Protein and mRNA level of CXCR3 in ITP group were 3.0 and 3.5 times as high as those in control group, respectively. Those of CCR5 in ITP group were 1.2 and 1.7 times as high as those in control group, respectively.
Conclusion: High expression of CXCR3 and CCR5 may play a part in the splenic immune disorders in patients with ITP.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
The Chemokine System as a Key Regulator of Pulmonary Fibrosis: Converging Pathways in Human Idiopathic Pulmonary Fibrosis (IPF) and the Bleomycin-Induced Lung Fibrosis Model in Mice.
Cells
December 2024
Laboratory of Immuno-Neuro Modulation (INEM), UMR7355 Centre National de la Recherche Scientifique (CNRS), University of Orleans, 45071 Orleans, France.
Idiopathic pulmonary fibrosis (IPF) is a chronic and lethal interstitial lung disease (ILD) of unknown origin, characterized by limited treatment efficacy and a fibroproliferative nature. It is marked by excessive extracellular matrix deposition in the pulmonary parenchyma, leading to progressive lung volume decline and impaired gas exchange. The chemokine system, a network of proteins involved in cellular communication with diverse biological functions, plays a crucial role in various respiratory diseases.
View Article and Find Full Text PDFFatty acids promote migration of CD4 T cells through calcium release-activated calcium modulator ORAI1 sensitive glycolysis in dairy cows.
J Dairy Sci
January 2025
College of Veterinary Medicine, China Agricultural University, Beijing 100000, China. Electronic address:
Nutritional and metabolic state in dairy cows are important determinants of the immune response. During the periparturient period, a state of negative energy balance in the cow increases plasma concentrations of fatty acids (FA), which are associated with inflammation. Among immune cells, CD4 T are able to function under high-FA conditions, but the underlying mechanisms regulating these events remain unclear.
View Article and Find Full Text PDFToll-Like Receptor 2 Attenuates the Formation and Progression of Angiotensin II-Induced Abdominal Aortic Aneurysm in ApoE-/- Mice.
J Vasc Res
December 2024
Molecular Cardiology Research Institute, Tufts Medical Center, Boston, Massachusetts, USA.
Introduction: We demonstrated Toll-like receptor (TLR) 4 in the pathogenesis of angiotensin II (AngII)-mediated abdominal aortic aneurysm (AAA) formation. Here, we study TLR2 in the AAA formation.
Methods: Male ApoE-/- and ApoE-/-TLR2-/- mice were treated with AngII.
Blood immune profiles reveal a CXCR3/CCR5 axis of dysregulation in early sepsis.
J Leukoc Biol
September 2024
Hull York Medical School, University of York.
We report on a pilot study exploring whether blood immune signatures can reveal early specific indicator profiles for patients meeting sepsis criteria upon hospital admission. We analysed samples of sepsis-suspected patients (N=20) and age-spanning healthy controls (N=12), using flow cytometry-based assays. We measured inflammatory markers from plasma fractions, and immunophenotyped freshly isolated unfixed PBMCs for leukocytes subsets representation and expression of activation markers, including chemokine receptors.
View Article and Find Full Text PDFIL23R mutations associated with decreased risk of psoriasis lead to the differential expression of genes implicated in the disease.
Exp Dermatol
September 2024
Manchester Academic Health Science Centre, Faculty of Biology Medicine and Health, School of Biological Sciences, The University of Manchester, Manchester, UK.
Article Synopsis
- * Researchers developed models using T cells to analyze how different forms of IL23R (wild type vs. protective mutant) impact the signaling pathway via STAT3, identifying genes related to psoriasis risk through RNA sequencing.
- * The study discovered several key genes (like HLA-B, SOCS1, and CCR5) that are differentially regulated based on IL23R form, contributing to a better understanding of the IL23 pathway and its role in psoriasis.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!