Maintenance of hematopoietic stem cells (HSCs) in vitro has been believed to be difficult due to a lack of complete understanding of HSC quiescence maintained by the niche. Recent evidence suggests that in vitro maintenance of human and mouse long-term HSCs (LT-HSCs) is possible through dual inhibition (2i) of both GSK-3 and mTOR in the absence of cytokines, serum, or feeder cells.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3616430 | PMC |
| http://dx.doi.org/10.1038/cr.2013.14 | DOI Listing |
Publication Analysis
Top Keywords
niche-less maintenance
4
maintenance hscs
4
hscs maintenance
4
maintenance hematopoietic
4
hematopoietic stem
4
stem cells
4
cells hscs
4
hscs vitro
4
vitro believed
4
believed difficult
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!