AI Article Synopsis
- A validated method for analyzing Cry proteins is essential for studying the environmental impact of genetically engineered Bacillus thuringiensis crops.
- The study optimized an extraction method for Cry1Ac protein in soil using a Box-Behnken experimental design, finding the best conditions at 21 °C and 630 rpm for 2 hours.
- This sensitive and precise method was successfully applied to field-collected soil samples, revealing trace amounts of Cry1Ac protein in soils where transgenic crops had been grown for 8 and 12 years.
Article Abstract
A validated method for analyzing Cry proteins is a premise to study the fate and ecological effects of contaminants associated with genetically engineered Bacillus thuringiensis crops. The current study has optimized the extraction method to analyze Cry1Ac protein in soil using a response surface methodology with a three-level-three-factor Box-Behnken experimental design (BBD). The optimum extraction conditions were at 21 °C and 630 rpm for 2 h. Regression analysis showed a good fit of the experimental data to the second-order polynomial model with a coefficient of determination of 0.96. The method was sensitive and precise with a method detection limit of 0.8 ng/g dry weight and relative standard deviations at 7.3%. Finally, the established method was applied for analyzing Cry1Ac protein residues in field-collected soil samples. Trace amounts of Cry1Ac protein were detected in the soils where transgenic crops have been planted for 8 and 12 years.
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| http://dx.doi.org/10.1021/jf304970g | DOI Listing |
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