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Development of an analytical approach to study a three-component hetero-association by means of spectrophotometry. | LitMetric
A case of 1:m:n complexation in a three-component system containing any possible heterocomplexes formed between the non-self-aggregating, absorbing ligand A and two self-aggregating, non-absorbing ligands B and C was considered for the first time in an application for molecular spectroscopy. All expressions necessary for full quantitative analysis of experimental data in three-component mixtures were obtained, viz., the law of conservation of mass and the expression for an experimentally observed parameter. These expressions can be directly utilized in mathematical software for performing standard curve-fitting procedures or solutions of specific tasks such as calculations of the concentration of various types of complexes. The numerical test of the 1:m:n model, accomplished with the aid of ultraviolet-visible light experimental data in a three-component system (proflavine-caffeine-nicotinamide), proved the validity of the developed approach.
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