We isolated 1,264 bacterial strains from forest soils previously surveyed via pyrosequencing of rRNA gene amplicons. Conventional culturing techniques recovered a substantial proportion of the community, with isolates representing 22% of 98,557 total pyrotags. Growth characteristics of isolates indicated that ecological traits were associated with relative abundances of corresponding pyrotag operational taxonomic units.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592214 | PMC |
| http://dx.doi.org/10.1128/AEM.03112-12 | DOI Listing |
Publication Analysis
Top Keywords
substantial proportion
8
isolation substantial
4
proportion forest
4
forest soil
4
soil bacterial
4
bacterial communities
4
communities detected
4
detected pyrotag
4
pyrotag sequencing
4
sequencing isolated
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!