Effect of Staphylococcus aureus and Staphylococcus epidermidis debris and supernatant on bone marrow stromal cells growth.

Aim: the goal of this study is to observe the effect of SA and SE debris and supernatant on BMSCs growth.

Methods: SA and SE were isolated and cultured from lesion materials of spondylitis patients which were determined by gram staining and biochemical tests. BMSCs were cultured and incubated for 11 days to be further sub-cultured and tripsinized for cell counting before seeded. 0.1 mg/ml SA and SE debris and supernatant were added into the BMSCs culture media. Cell counting was performed 2, 5, 7, and 9 days after debris and supernatant addition to get the growth profile of BMSCs.

Results: debris and supernatant decreases BMSCS number at initial day. At day 5, BMSCs in the group debris were growing down, mean while BMSCs in the group supernatant were able to retain the cell number. Viability of all groups was more than 80%.

Conclusion: both debris and supernatant from SA and SE have inhibitory effect of the growth BMSCs in the initial day. BMSCs could provide barrier to survive from the debris and supernatant environment of both bacteria in day five. BMSCs can use supernatant to retain the growth to replace the lack of nutrition.