Motivation: A common question arises at the beginning of every experiment where RNA-Seq is used to detect differential gene expression between two conditions: How many reads should we sequence?
Results: Scotty is an interactive web-based application that assists biologists to design an experiment with an appropriate sample size and read depth to satisfy the user-defined experimental objectives. This design can be based on data available from either pilot samples or publicly available datasets.
Availability: Scotty can be freely accessed on the web at http://euler.bc.edu/marthlab/scotty/scotty.php
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3582267 | PMC |
| http://dx.doi.org/10.1093/bioinformatics/btt015 | DOI Listing |
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