To study the attachment, proliferation and differentiation of neural stem cells (NSCs) on surface modified PHBHHx films and to establish the theory of PHBHHx application in NSCs-based brain tissue engineering. PHBHHx film was fabricated by a solution-casting method, and the morphology of the film was observed under scanning electron microscopy(SEM). The films were treated by NaOH or lipase, then the surface hydrophilic property was characterized using water contact angle measurement. NSCs were isolated from the cerebral cortex of rat embryos on embryonic day 14.5, and cultured on surface treated PHBHHx films. The morphology of NSCs attached on the film was visualized under SEM, and the survival and differentiation of NSCs were observed through immunocytochemical staining. Compared with the untreated PHBHHx films, the water contact angle of NaOH or lipase treated PHBHHx films decreased dramatically, and the number of NSCs attached significantly increased. NSCs survived well on treated PHBHHx films and differentiated into neurons and glial cells. The amelioration of hydrophilic property of PHBHHx film improved its biocompatibility with NSCs. PHBHHx can serve as a novel CNS tissue engineering biomaterial applied for NSCs transplantation, brain repairing and regeneration.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Evaluation of Blended Poly(3-hydroxybutyrate--3-hydroxyhexanoate) Properties Containing Various 3HHx Monomers.
Polymers (Basel)
October 2024
Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Republic of Korea.
Article Synopsis
- - Polyhydroxyalkanoate (PHA), particularly P(3HB--3HHx), is a bioplastic whose properties are influenced by the mole fraction of 3-hydroxyhexanoate (3HHx) but faces challenges in mass production due to complex fermentation conditions and strain development.
- - To address production obstacles, researchers blended poly(3-hydroxybutyrate) (P(3HB)) with different 3HHx content films, assessing their molecular weight, thermal, mechanical, and degradability properties.
- - The study found that these blended films maintained similar properties to microbial P(3HB--3HHx) and demonstrated a high degradation rate, suggesting that the simple blending method
Fabrication of Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)/ZnO Nanocomposite Films for Active Packaging Applications: Impact of ZnO Type on Structure-Property Dynamics.
Polymers (Basel)
June 2024
Materials and Packaging Research & Services, Institute for Materials Research (IMO-IMOMEC), Hasselt University, Wetenschapspark 27, 3590 Diepenbeek, Belgium.
Bio-based and biodegradable polyhydroxyalkanoates (PHAs) have great potential as sustainable packaging materials. The incorporation of zinc oxide nanoparticles (ZnO NPs) could further improve their functional properties by providing enhanced barrier and antimicrobial properties, although current literature lacks details on how the characteristics of ZnO influence the structure-property relationships in PHA/ZnO nanocomposites. Therefore, commercial ZnO NPs with different morphologies (rod-like, spherical) and silane surface modification are incorporated into poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) via extrusion and compression molding.
View Article and Find Full Text PDFA bionanocomposite of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)/ZnO-nanoparticles intended for food packaging.
Int J Biol Macromol
May 2023
Department of Mathematics and Sciences, Faculty of Humanities and Sciences, Prince Sultan University, Riyadh, Saudi Arabia. Electronic address:
Films-based bionanocomposites have gained a great importance in food plastic packaging because they are eco-friendly materials and have the potential to improve food protection, while limiting the accumulation of synthetic plastics on the planet. In this paper, biofilms were prepared using poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) reinforced with Zinc oxide nanoparticles (ZnO-NPs) to develop new bionanocomposite materials intended for food packaging. The samples were fabricated using first solvent casting method followed by melt compounding at various loading rates, i.
View Article and Find Full Text PDFOxygen Gas and UV Barrier Properties of Nano-ZnO-Coated PET and PHBHHx Materials Fabricated by Ultrasonic Spray-Coating Technique.
Nanomaterials (Basel)
February 2021
IMO-IMOMEC, Packaging Technology Center, Hasselt University, Wetenschapspark 27, 3590 Diepenbeek, Belgium.
Ultrasonic spray-coating (USSC)-a wet chemical deposition method to deposit ultrathin (down to 20 nm) coatings-is being applied as a promising alternative deposition method for functional coatings due to an economical, simple, and precise coating process with easy control over its operating parameters. In this research, zinc oxide nanoparticles (ZnO NPs) were ultrasonically spray-coated on commercial-grade polyethylene terephthalate (PET) and poly(3-hydroxybutyrate--3-hydroxyhexanoate) (PHBHHx) films. The most suitable parameters for the ink composition, the ultrasonic spray-coating process, and the number of coating passes (up to 50×) were selected on the basis of a series of experiments.
View Article and Find Full Text PDFSuperhydrophobic Polyhydroxyalkanoates: Preparation and Applications.
Biomacromolecules
February 2019
Poly( R-3-hydroxybutyrate- co- R-3-hydroxyhexanoate) (PHBHHx), a family member of microbial polyhydroxyalkanoates (PHA), is a biodegradable and biocompatible material with some hydrophobicity and reasonable strength for packaging and tissue engineering applications. In this study, superhydrophobic PHBHHx is fabricated via a simple nonsolvent-assisted process. The material can absorb all tested hydrophobic solvents and oil up to 6-fold of the material weights from water, permitting applications for cleaning environmental oil or solvent pollutions with convenience of disposal after the usage due to its biodegradability.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!