A highly specific lead-binding peptide ThrAsnThrLeuSerAsnAsn was displayed on Escherichia coli, and lead adsorption characteristics of the recombinant bacteria were investigated. Cell surface-displayed peptide was expressed under the control of an arabinose promoter using outer membrane protein C (OmpC(t)) as an anchoring motif. The optimal induction period and arabinose concentration for the expression of peptide-fused OmpC(t) were determined to be 2 h and 0.001 g/L, respectively. Selective adsorption of Pb(2+) onto recombinant cells was verified with individual or combinatory use of four metal ions, Pb(2+), Ni(2+), Co(2+), and Cu(2+); the amount of bound Pb(2+) onto the biosorbents was significantly higher than the other metal ions. The adsorption isotherm of recombinant cells for Pb(2+) followed the Langmuir isotherm with a maximum adsorption loading (q (max)) of 526 μmol/g dry cell weight.
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