Production and partial characterization of cellulases and Xylanases from Trichoderma atroviride 676 using lignocellulosic residual biomass. | LitMetric
Departamento de Microbiologia Geral, Instituto de Microbiologia Prof. Paulo de Góes, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-590, Brazil.
Published: February 2013
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Article Abstract
Trichoderma atroviride 676 was studied to evaluate its efficiency in the production of some lignocellulolytic enzymes, using lignocellulosic residual biomass. Best results were obtained when 3.0 % (w/v) untreated sugarcane bagasse was used (61.3 U mL(-1) for xylanase, 1.9 U mL(-1) for endoglucanase, 0.25 U mL(-1) for FPase, and 0.17 U mL(-1) for β-glucosidase) after 3-4 days fermentation. The maximal enzymatic activity for endoglucanase, FPase, and xylanase were observed at 50-60 °C and pH 4.0-5.0, whereas thermal stability at 50 °C (CMCase and FPase) or 40 °C (xylanase) was obtained after 8 h. Zymograms have shown two bands of 104 and 200 kDa for endoglucanases and three bands for xylanase (23, 36, and 55.7 kDa). The results obtained with T. atroviride strain 676 were comparable to those obtained with the cellulolytic strain Trichoderma reesei RUT-C30, indicating, in the studied conditions, its great potential for biotechnological application, especially lignocellulose biomass hydrolysis.
, the pathogen responsible for apple replant disease (ARD), is seriously threatening the apple industry globally. We investigated the antagonistic properties of strains against HS2, aiming to find a biological control solution to minimize the dependence on chemical pesticides. Two of the thirty-one strains assessed through plate confrontation assays, L7 () and M19 (), markedly inhibited = , with inhibition rates of 86.
Multidisciplinary Center for Biotechnology Studies, Faculty of Veterinary Medicine and Zootechnics, Universidad Michoacana de San Nicolás de Hidalgo, Km 9.5 Carretera Morelia-Zinapécuaro, Col. La Palma, Tarímbaro CP 58893, Michoacán, Mexico.
This work describes the characterization of strain CMU-08, isolated from Michoacán, Mexico. CMU-08 demonstrated robust growth and conidiation across a temperature range from 16 to 32 °C and a pH range from 4 to 9 on potato dextrose agar (PDA) and malt extract agar (MEA) media. The strain is an efficient antagonist of six species of phytopathogenic fungi and oomycetes in PDA, MEA, and Vogel minimal medium (VMM).
The need to increase yield and enhance the sustainability of crop production systems has led to the development and employment of natural products, such as plant biostimulants. In recent years, a number of reports have researched the effects of biostimulants on plant performance; however, few studies have focused on the mutual application of microbial and/or non-microbial biostimulants. This research, conducted in the framework of the SO.
Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Rosario (UNR), Suipacha 531, Rosario 2000, Santa Fe, Argentina.
Symbiosis establishment between and plant roots triggers the priming of defense responses, among other effects. Currently, there is no clear evidence regarding the molecular mechanisms that allow the plant to remain alert to future stimulus, either by pathogen attack or any other abiotic stress. Epigenetic modifications have emerged as a strategy to explain the increased defense response of plants in a priming state conferred by .
Parasitic species of use hydrolytic enzymes to destroy the host cell wall. Preferent carbon and nitrogen sources suppress the expression of genes related to parasitism. Here, different nutrients were evaluated in the parasitic isolated NI-1, which was identified as .
