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- * MET expression peaks during critical stages of development when 20E levels are high, and knocking down MET disrupts important transcriptional pathways, leading to failed tissue remodeling and subsequent lethality.
- * The findings suggest MET interacts physically with the ecdysone receptor (EcR-USP) and forms a complex that is necessary for 20E-induced gene transcription, highlighting its importance during the silkworm's metamorphic transition.
Article Abstract
Little is known about how the putative juvenile hormone (JH) receptor, the bHLH-PAS transcription factor MET, is involved in 20-hydroxyecdysone (20E; the molting hormone) action. Here we report that two MET proteins found in the silkworm, Bombyx mori, participate in 20E signal transduction. Met is 20E responsive and its expression peaks during molting and pupation, when the 20E titer is high. As found with results from RNAi knockdown of EcR-USP (the ecdysone receptor genes), RNAi knockdown of Met at the early wandering stage disrupts the 20E-triggered transcriptional cascade, preventing tissue remodeling (including autophagy, apoptosis and destruction of larval tissues and generation of adult structures) and causing lethality during the larval-pupal transition. MET physically interacts with EcR-USP. Moreover, MET, EcR-USP and the 20E-response element (EcRE) form a protein-DNA complex, implying that MET might modulate 20E-induced gene transcription by interacting with EcR-USP. In conclusion, the 20E induction of MET is required for the maximal action of 20E during Bombyx metamorphosis.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3531340 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0053256 | PLOS |
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