AI Article Synopsis
- * The authors present a new method for predicting EF-hand motifs in viral genomes using their primary sequences, which enhances our understanding of viral Ca(2+) interactions.
- * A novel grafting technique and experimental methods, including luminescence resonance energy transfer and Ca(2+) competition assays, are introduced for verifying these predicted binding sites, aiding in the identification of previously unknown Ca(2+)-binding sites in viruses.
Article Abstract
Ca(2+) is implicated in almost every step of the life cycle of viruses, including virus entry into host cells, virus replication, virion assembly, maturation, and release. However, due to the lack of prediction algorithms and rigorous validation methods, only limited cases of viral Ca(2+)-binding sites are reported. Here, we introduce a method to predict continuous EF-hand or EF-hand-like motifs in the viral genomes based on their primary sequences. We then introduce a grafting approach, and the use of luminescence resonance energy transfer and Ca(2+) competition assay for experimental verification of predicted Ca(2+)-binding sites. This protocol will be valuable for the prediction and identification of unknown Ca(2+)-binding sites in virus.
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| http://dx.doi.org/10.1007/978-1-62703-230-8_3 | DOI Listing |
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