The high salinity and fat contents of kitchen waste (KW) inhibits the effect of two-phase anaerobic digestion system. This research introduces fruit-vegetable waste (FVW) to alleviate the inhibition effect caused by salinity and fat concentrations, and tries to achieve an optimal addition ratio of FVW, an optimal hydraulic remain time (HRT) of acidogenic-phase reactor and methanogenic-phase reactor. A two-phase anaerobic digestion (AD) system was developed to co-dispose KW and FVW. Four sets of experiments were run with different mass proportions between KW and FVW (25-75, 50-50, 75-25, and 100-0% m/m). Considering the biodegradation rate and the acidification degree, the system with 25% KW had the best performance during the acidogenic phase. When the system was run with 50% KW, it not only had the best stability performance but also had a bigger capacity to treat KW than the system with 25% KW. The system with 50% KW was the best ratio in this two-phase AD system. Co-digestion of KW and FVW by two-phase AD is feasible. The addition of FVW can reduce the inhibition effect caused by salinity and fat concentrations, reduce the HRT, and lead to a higher degree of acidification.
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http://dx.doi.org/10.1007/s11356-012-1414-y | DOI Listing |
Biotechnol Bioeng
December 2024
Department of Civil and Environmental Engineering, University of Michigan, Ann Arbor, Michigan, USA.
An open-source modeling platform, called Anaerobic Digestion Model No. 1 Fast (ADM1F), is introduced to achieve fast and numerically stable simulations of anaerobic digestion processes. ADM1F is compatible with an iPython interface to facilitate model configuration, simulation, data analysis, and visualization.
View Article and Find Full Text PDFInt J Syst Evol Microbiol
December 2024
Department of Fermentation Science, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo, 156-8502, Japan.
An obligately anaerobic, Gram-stain-negative, non-spore-forming and non-motile rod (strain LPYR103-Pre) was isolated from a two-phase methane fermentation system. Using 16S rRNA gene sequence-based phylogenetic analysis, strain LPYR103-Pre was classified in the genus . The 16S rRNA gene sequence similarity, average nucleotide identity and digital DNA-DNA hybridization between strain LPYR103-Pre and its phylogenetically nearest species JCM 30867 - were 94.
View Article and Find Full Text PDFBioresour Technol
December 2024
School of Engineering and Technology, Central Michigan University, Mount Pleasant, MI, USA; Institute for Great Lakes Research, Central Michigan University, Mount Pleasant, MI, USA. Electronic address:
Two-phase anaerobic digestion (AD) performance is significantly influenced by operating parameters such as temperature and solids retention time (SRT), while their impact on antibiotic resistance genes (ARGs) during the acidogenic (AP) and methanogenic (MP) phases remains unclear. This study assessed the abundance of eight ARGs in full-scale two-phase AD, then operated lab-scale two-phase AD systems to evaluate temperature combinations (thermophilic-thermophilic, thermophilic-mesophilic, mesophilic-thermophilic, and mesophilic-mesophilic) at a constant SRT (AP = 2/MP = 13d) and to further assess different SRTs (AP = 2/MP = 13d and AP = 4/MP = 11d). qPCR results revealed that full-scale two-phase AD reduced total ARGs abundance by 87.
View Article and Find Full Text PDFAngew Chem Int Ed Engl
November 2024
Center of Basic Molecular Science (CBMS), Department of Chemistry, Tsinghua University, Beijing, 100084, China.
Perchlorate, initially regarded as a weakly coordinating counterion rather than a reactive oxidizing reagent due to its kinetic stability, has garnered attention for its potential in microbial systems. Under anaerobic conditions, microbes utilize perchlorate as a terminal oxidant for methane oxidation, involving two distinct stages: extraction and release of oxidizing ability. This two-phase activation process necessitates the collaborative action of multiple enzymes, a phenomenon not extensively explored in artificial systems.
View Article and Find Full Text PDFSci Total Environ
December 2024
Key Laboratory of Biotechnology and Bioresources Utilization, Ministry of Education, Dalian Minzu University, 18 Liaohe Road West, Dalian Economic and Technological Development Reactor, Dalian 116600, China; College of Environment and Resources, Dalian Minzu University, 18 Liaohe West Road, Dalian 116600, China.
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