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Intercellular mRNA transfer alters the human pluripotent stem cell state.
Proc Natl Acad Sci U S A
January 2025
Human Biology Research Unit, Institute of Integrated Research, Institute of Science Tokyo, Bunkyo-ku, Tokyo 113-8510, Japan.
Intercellular transmission of messenger RNA (mRNA) is being explored in mammalian species using immortal cell lines. Here, we uncover an intercellular mRNA transfer phenomenon that allows for the adaptation and reprogramming of human primed pluripotent stem cells (hPSCs). This process is induced by the direct cell contact-mediated coculture with mouse embryonic stem cells under the condition impermissible for primed hPSC culture.
View Article and Find Full Text PDFDoes Virtual Interviewing Provide the Information for a Satisfactory Rank Decision?: A Perspective From the Pediatric Emergency Medicine Fellowship Interviews.
Pediatr Emerg Care
January 2025
From the Pediatric Emergency Medicine, UT Southwestern Medical Center, Children's Health, Dallas, TX.
Objective: The primary aim of this study was to determine whether current fellows and program directors in pediatric emergency medicine (PEM) were satisfied with virtual interviewing (VI) in terms of their respective matches. The secondary goal was to assess areas in which the virtual interview process could be improved.
Methods: After institutional review was obtained, fellow surveys were piloted to non-PEM fellows to solicit feedback.
Bacteriological diagnosis of osteoarticular infections caused by ; a narrative review.
Front Pediatr
January 2025
Pediatric Orthopedic Unit, Pediatric Surgery Service, Geneva University Hospitals, Geneva, Switzerland.
In recent years, advancements in modern laboratory diagnostics have identified () as the major cause of osteoarticular infections in early childhood. The introduction of novel diagnostic methods has ushered in a new era, transitioning from underrated infections to recognizing as the primary etiology of skeletal system infections in children. This article provides a new perspective on , exploring innovative diagnostic methods that have improved and will continue to transform the management of these infections.
View Article and Find Full Text PDFCurrent status and perspectives of hematopoietic cell transplantation in patients with paroxysmal nocturnal hemoglobinuria.
Front Immunol
January 2025
Department of Hematology and Transplantology, Medical University of Gdansk, Gdansk, Poland.
Background: Paroxysmal nocturnal hemoglobinuria (PNH) is a rare complement-driven acquired hemolytic anemia with specific presentations of hemoglobinuria, abdominal pain, fatigue, and thrombosis.
Objective: To review the current therapeutic strategies for PNH, including anti-complement therapy and allogeneic hematopoietic cell transplantation (alloHCT), focusing on the tailoring of the approach to the disease subtype.
Results: The outcome of alloHCT varies depending on disease severity, thrombotic history, and response to prior therapies.
Breathing Easy: A Qualitative Analysis of School Nurse and Administrator Perspectives on Stock Inhalers and School-Based Asthma Management.
J Sch Nurs
January 2025
Department of Pediatrics, University of Illinois Chicago College of Medicine, Chicago, IL, USA.
Stock inhalers are unassigned rescue inhalers available for students who experience asthma symptoms at school. This study sought to understand school nurse and administrator perspectives on school-based asthma management and stock inhaler policy implementation. Twenty-three semistructured interviews were conducted with professionals from five high asthma burdened counties in varied geographic settings.
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