AI Article Synopsis
- * A study assessed immune responses in different groups, including active-TB patients and health care workers, using the QuantiFERON -TB Gold In-Tube test alongside flow cytometry.
- * Results showed a strong correlation between IFN-γ levels and certain CD4 T cell frequencies, with active-TB patients having significantly higher CD4+/CD69+/IFN-γ+ T cell counts than other groups, and a notable response in CD8 T cells among recent contacts of TB patients.
Article Abstract
Current diagnostic standards for Mycobacterium tuberculosis (MTB) infection do not distinguish between active and latent tuberculosis (TB). To identify specific biomarkers characterizing the different forms of TB infection, we investigated in parallel with the QuantiFERON -TB Gold In-Tube (QFT-IT) the use of flow cytometry measuring CD4 and CD8 MTB-specific immune response in 17 active-TB patients, 21 health care workers (HCW), 14 recent contacts of TB patients (RC-TB), and 10 bacille Calmette Guerin (BCG)-vaccinated healthy controls (BCG-HC). A correlation (r = 0.4526, P = 0.0002) was found only between the amount of IFN-γ measured by QFT-IT and the frequency of CD4+/CD69+/IFN-γ+ T cells. The frequency of CD4+/CD69+/IFNγ+ responding T cells was higher in active-TB patients (0.254 ± 0.336%, P < 0.01) compared to the other groups. The response of QFT-IT antigen-specific CD8+/CD69+/IFNγ+ T cells was significantly higher in RC-TB (0.245 ± 0.305%, P < 0.05) compared to the other study groups.
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| http://dx.doi.org/10.1016/j.diagmicrobio.2012.11.023 | DOI Listing |
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