Endophytes are microorganisms that asymptomatically invade plant tissues. They can stimulate plant growth and/or provide defense against pathogen attacks through the production of secondary metabolites. Most endophyte species are still unknown, and because they may have several applications, the study of their metabolic capabilities is essential. We characterized 100 endophytes isolated from Espeletia spp., a genus unique to the paramo ecosystem, an extreme environment in the Andean mountain range. We evaluated the cellulolytic potential of these endophytes on the saccharification of the oil palm empty fruit bunch (OPEFB). The total cellulolytic activity was measured for each endophyte on filter paper (FPA). In addition, the specific carboxymethyl cellulase (CMCase), exoglucanase, and β-glucosidase activities were determined. We found four fungi positive for cellulases. Of these fungi, Penicillium glabrum had the highest cellulolytic activity after partial purification, with maximal CMCase, exoglucanase and β-glucosidase enzyme activities of 44.5, 48.3, and 0.45 U/ml, respectively. Our data showed that the bioprospection of fungi and the characterization of their enzymes may facilitate the process of biofuel production.
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http://dx.doi.org/10.1007/s12275-012-2130-5 | DOI Listing |
Chem Biodivers
December 2024
North-West University - Mafikeng Campus, Indigenous Knowledge Systems Centre, G10, 2735, Mmabatho, SOUTH AFRICA.
The genus Grewia are known for their medicinal properties. We isolated and characterized five endophytic fungi from Grewialasiocarpa for in vitro antibacterial and antioxidant activities. Five [Aspergillus fumigatus (MK243397.
View Article and Find Full Text PDFNat Prod Res
December 2024
Guangxi Key Laboratory of Bioactive Molecules Research and Evaluation & Guangxi Health Commission Key Laboratory of Basic Research on Antigeriatric Drugs, College of Pharmacy, Guangxi Medical University, Nanning, China.
Studies on the chemical composition of endophytic fungus , isolated from the L., by using epigenetic modification to activate clusters of biosynthetic genes that are silenced or poorly expressed in fungi, resulting in obtainment of ten polyketides. Among these compounds were six new compounds, including cytosporin E2 (), cytosporin D1 (), cytosporin G1 (), cytosporin J1 (), cytosporin K1 (), cytosporin F1 (), and four known compounds, cytosporin E (), cytosporin Y3 (), cytosporin D (), and cytosporin L ().
View Article and Find Full Text PDFAMB Express
December 2024
Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Cairo, 11566, Egypt.
Antimicrobial resistance (AMR) represents a critical public health issue that requiring immediate action. Wild halophytic plants can be the solution for the AMR crisis because they harbor unique endophytes capable of producing potent antimicrobial metabolites. This study aimed at identifying promising and antimicrobial metabolites produced by endophytic/epiphytic bacteria recovered from the wild Bassia scoparia plant.
View Article and Find Full Text PDFCommun Integr Biol
December 2024
Central Department of Botany, Tribhuvan University, Kirtipur, Kathmandu, Nepal.
We isolated endophytic strains from the healthy roots, stems, and leaves of to investigate their plant growth-promoting activities in vitro. Subsequently, Indole acetic acid (IAA) was quantified and the gene (responsible for IAA synthesis in fungi) was amplified and sequenced. Finally, a germination assay was performed with seeds of and a plant growth assay with protocorms of to test their plant growth-promoting activities.
View Article and Find Full Text PDFJ Agric Food Chem
December 2024
School of Chemistry, Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, South China Normal University, Guangzhou 510006, China.
Three previously undescribed steroid-polyketone conjugates, talarergosteroids A-C (-), together with talarergosteroid D (), which was first identified from a natural source, were isolated from a derived fungus sp. SCNU-F0041. Compounds and bear a complicated 6/6/6/5/6/6 hexacyclic ring system characterized by an oxaspiro[5.
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