A multiplex PCR (mPCR) protocol was developed for simultaneous detection of the gyrB gene in Streptococcus pneumoniae, Streptococcus mitis, and Streptococcus oralis, and the specificity was evaluated using 141 coccus strains. Genomic DNAs purified from S. pneumoniae, S. mitis, and S. oralis strains were efficiently detected with size differences, whereas no PCR products were amplified from any of the reference strains tested. A pilot study of 47 human oral swab specimens was conducted in parallel, and the mPCR assay identified S. pneumoniae in 1 sample, S. mitis in 8 samples, and S. oralis in 2 samples, providing a powerful means for characterization at the level of species compared with traditional culture analysis. Our results suggest that the mPCR protocol presented here is a sensitive and promising tool for the rapid detection and discrimination of S. pneumoniae, S. mitis, and S. oralis from clinical specimens.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3592088 | PMC |
| http://dx.doi.org/10.1128/JCM.02920-12 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Metagenomic Analysis of the Buccal Microbiome by Nanopore Sequencing Reveals Structural Differences in the Microbiome of a Patient with Molar Incisor Hypomineralization (MIH) Compared to a Healthy Child-Case Study.
Int J Mol Sci
December 2024
Department of Medical and Molecular Biology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 19 Jordana St., 41-808 Zabrze, Poland.
Molar incisor hypomineralization (MIH) is a qualitative developmental defect that affects the enamel tissue of permanent molars and can also occur in permanent incisors. Enamel affected by MIH has reduced hardness, increased porosity, and a higher organic content than unaffected enamel. These characteristics predispose the enamel to accumulation of bacteria and a higher prevalence of caries lesions.
View Article and Find Full Text PDFRespiratory pathogens carriage in guinea pigs raised as livestock in Ecuador: A proxy to study a neglected reservoir for zoonotic transmission in the Andean Region.
Acta Trop
December 2024
One Health Research Group. Universidad de Las Américas. Quito. Ecuador. Electronic address:
In the Andean region of South America, guinea pigs (Cavia porcellus) are raised as livestock in close contact with other domestic animals and humans. Although the susceptibility of guinea pigs to being affected by a wide range of diseases is well known as a laboratory animal model, there are a few reports about the potential role of zoonotic pathogens in livestock guinea pigs. In this work, we analyzed the nasopharyngeal carriage of respiratory pathogenic viruses and bacteria in guinea pigs from farms in Ecuador.
View Article and Find Full Text PDFA novel LAMP-based assay for the identification of and in clinical isolates.
J Clin Microbiol
December 2024
Division of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden.
Article Synopsis
- * Identifying specific species within SMG is challenging due to their genetic similarities, making traditional testing methods (like phenotypic tests and MALDI-TOF) unreliable.
- * This study introduced a new method called LAMP (loop-mediated isothermal amplification) that uses unique molecular markers for accurate and quick identification of relevant SMG clinical isolates.
Clinical relevance of lung microbiota composition in critically ill children with acute lower respiratory tract infections: insights from a retrospective analysis of metagenomic sequencing.
Eur J Clin Microbiol Infect Dis
November 2024
Pediatric Intensive Care Unit, Children's Hospital of Soochow University, No. 92, Zhongnan Street, Suzhou Industrial park, Suzhou, Jiangsu, 215025, China.
Purpose: Acute lower respiratory tract infections (ALRIs) is a leading cause of child mortality worldwide. Metagenomic next-generation sequencing (mNGS) identifies ALRIs pathogens and explores the lung microbiota's role in disease severity and clinical outcomes. This study examines the association between lung microbiota and ALRIs outcomes in children, exploring its potential as a prognostic biomarker.
View Article and Find Full Text PDFMultiomics Analysis Reveals Significant Disparities in the Oral Microbiota and Metabolites Between Pregnant Women with and without Periodontitis.
Infect Drug Resist
October 2024
Cuiying Biomedical Research Center, Lanzhou University Second Hospital, Lanzhou, Gansu, 730030, People's Republic of China.
Article Synopsis
- The study explored the differences in oral microbiota and metabolites in pregnant women with periodontitis compared to those without it.
- Researchers collected subgingival plaque samples for comprehensive genomic sequencing and metabolomic analysis, revealing significant microbial and metabolic differences between the two groups.
- Findings suggest potential biomarkers for identifying periodontitis during pregnancy and provide insights for future clinical interventions.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!