AI Article Synopsis
- Angiogenesis is vital in the early stages of tumor development and progression, making it important to study and inhibit.
- A zebrafish model was created to investigate angiogenesis and screen for new anti-angiogenic drugs, using glioma U87 cells that express a red fluorescent protein.
- The study found that TGF-β1 promotes glioma-induced angiogenesis through the JNK pathway, and this process involves macrophage infiltration, highlighting the model's potential for understanding tumor-related angiogenesis and drug discovery.
Article Abstract
Angiogenesis plays a crucial role at the early stage of tumorigenesis and tumor progression. A suitable model will be useful not only for the clarification of the underlying molecular mechanisms, but also for high-throughput identification of novel anti-angiogenesis compounds. Here, we established a zebrafish model for the purpose to investigate angiogenesis and screen anti-angiogenic compounds. Glioma U87 cells expressing red fluorescent protein (RFP) were transplanted in fli:GFP transgenic zebrafish embryos where significant angiogenesis was observed. TGF-β1 enhanced glioma-induced angiogenesis, which was inhibited by JNK inhibitor SP600125 but not p38 MAPK inhibitor SB202190, ERK inhibitor PD98059, or PI3K inhibitor LY294002, indicating the important role of TGF-β1 and JNK pathways in this process. Moreover, the glioma-induced angiogenesis was associated with macrophage infiltration that was further enhanced by TGF-β1. Therefore, our zebrafish model provides a powerful in vivo tool for the investigation of tumor-induced angiogenesis, and a cost-effective system for high-throughput screening of anti-angiogenic compounds.
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| http://dx.doi.org/10.1016/j.intimp.2012.12.002 | DOI Listing |
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