To develop a chemical stimulus-responsive substrate for culturing cells, polyethyleneimine (PEI) having a pyridyl disulfide moiety was attached via disulfide linkages to a glass coverslip modified with a silane coupling agent having a thiol group. The surface modification was confirmed by X-ray photoelectron spectroscopy and zeta potential analysis. The obtained surface exhibited sufficiently high cell adhesiveness. Zeta potential measurements revealed that the PEI derivatives were released from the surface through thiol-disulfide exchange when the modified glass coverslip was immersed in a neutral pH buffer containing cysteine. The cell viability assay demonstrated that this chemical stimulus was substantially nontoxic to 293T cells. Because PEI is a widely used transfection reagent, this functional glass coverslip would be potentially useful as an experimental platform for reverse transfection.
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